caa a22 4500 528786369 20190713030956.0 cr unu---uuuuu 180924s2016 xx s 000 0 eng 10.3929/ethz-b-000122781 doi 10.1038/ncomms13631 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/122781 Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles [Elektronische Daten] [Patrick Studer, Titu Staubli, Noémie Wieser, Patrick Wolf, Markus Schuppler, Martin J. Loessner] Nat Commun Open access ethresearch L-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein FtsZ. Here we describe FtsZ-independent mechanisms, involving internal and external vesicles, by which Listeria monocytogenes L-forms proliferate. Using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, receive cytoplasmic content, and represent viable progeny. In addition, the L-forms can reproduce by pearling, that is, generation of extracellular vesicles that remain transiently linked to their mother cell via elastic membranous tubes. Using photobleaching and fluorescence recovery, we demonstrate cytoplasmic continuity and transfer through these membranous tubes. Our findings indicate that L-forms' polyploidy and extended interconnectivity through membranous tubes contribute to the generation of viable progeny independently of dedicated division machinery, and further support L-forms as models for studies of potential multiplication mechanisms of hypothetical primitive cells. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Studer Patrick joint author Staubli Titu joint author Wieser Noémie joint author Wolf Patrick joint author Schuppler Markus joint author Loessner Martin J. joint author Nature Communications London : Nature Publishing Group 7, p. 13631 2041-1723 http://hdl.handle.net/20.500.11850/122781 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/122781 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Studer Patrick joint author ETHRESEARCH 700 1- Staubli Titu joint author ETHRESEARCH 700 1- Wieser Noémie joint author ETHRESEARCH 700 1- Wolf Patrick joint author ETHRESEARCH 700 1- Schuppler Markus joint author ETHRESEARCH 700 1- Loessner Martin J. joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 7, p. 13631 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access