caa a22 4500 52878658X 20181217030316.0 cr unu---uuuuu 180924e20151126xx s 000 0 eng 10.3929/ethz-b-000108322 doi 10.1038/srep17180 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/108322 Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S-pombe cells [Elektronische Daten] [Zhen Zhu, Olivier Frey, Niels Haandbaek, Felix Franke, Fabian Rudolf, Andreas; id_orcid 0000-0002-3838-2468 Hierlemann] Sci Rep Open access ethresearch As a complement and alternative to optical methods, wide-band electrical impedance spectroscopy (EIS) enables multi-parameter, label-free and real-time detection of cellular and subcellular features. We report on a microfluidics-based system designed to reliably capture single rod-shaped Schizosaccharomyces pombe cells by applying suction through orifices in a channel wall. The system enables subsequent culturing of immobilized cells in an upright position, while dynamic changes in cell-cycle state and morphology were continuously monitored through EIS over a broad frequency range. Besides measuring cell growth, clear impedance signals for nuclear division have been obtained. The EIS system has been characterized with respect to sensitivity and detection limits. The spatial resolution in measuring cell length was 0.25 μm, which corresponds to approximately a 5-min interval of cell growth under standard conditions. The comprehensive impedance data sets were also used to determine the occurrence of nuclear division and cytokinesis. The obtained results have been validated through concurrent confocal imaging and plausibilized through comparison with finite-element modeling data. The possibility to monitor cellular and intracellular features of single S. pombe cells during the cell cycle at high spatiotemporal resolution renders the presented microfluidics-based EIS system a suitable tool for dynamic single-cell investigations. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Lab-on-a-chip ethresearch Biomedical engineering ethresearch Zhu Zhen joint author Frey Olivier joint author Haandbaek Niels joint author Franke Felix joint author Rudolf Fabian joint author Hierlemann Andreas; id_orcid 0000-0002-3838-2468 joint author Scientific Reports London : Nature Publishing Group 5, p. 17180 2045-2322 http://hdl.handle.net/20.500.11850/108322 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/108322 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Zhu Zhen joint author ETHRESEARCH 700 1- Frey Olivier joint author ETHRESEARCH 700 1- Haandbaek Niels joint author ETHRESEARCH 700 1- Franke Felix joint author ETHRESEARCH 700 1- Rudolf Fabian joint author ETHRESEARCH 700 1- Hierlemann Andreas; id_orcid 0000-0002-3838-2468 joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 5, p. 17180 2045-2322 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access