naa a22 4500 528786903 20180924065502.0 cr unu---uuuuu 180924e20120712xx s 000 0 eng 10.3929/ethz-b-000058663 doi 10.1371/journal.pone.0040526 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/58663 A Meta-Analysis Reveals the Commonalities and Differences in Arabidopsis thaliana Response to Different Viral Pathogens [Elektronische Daten] [Guillermo Rodrigo, Javier Carrera, Virgina Ruiz-Ferrer, Francisco J. Del Toro, César Llave, Olivier Voinnet, Santiago F. Elena] PLoS ONE Open access ethresearch Understanding the mechanisms by which plants trigger host defenses in response to viruses has been a challenging problem owing to the multiplicity of factors and complexity of interactions involved. The advent of genomic techniques, however, has opened the possibility to grasp a global picture of the interaction. Here, we used Arabidopsis thaliana to identify and compare genes that are differentially regulated upon infection with seven distinct (+)ssRNA and one ssDNA plant viruses. In the first approach, we established lists of genes differentially affected by each virus and compared their involvement in biological functions and metabolic processes. We found that phylogenetically related viruses significantly alter the expression of similar genes and that viruses naturally infecting Brassicaceae display a greater overlap in the plant response. In the second approach, virus-regulated genes were contextualized using models of transcriptional and protein-protein interaction networks of A. thaliana. Our results confirm that host cells undergo significant reprogramming of their transcriptome during infection, which is possibly a central requirement for the mounting of host defenses. We uncovered a general mode of action in which perturbations preferentially affect genes that are highly connected, central and organized in modules. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Rodrigo Guillermo joint author Carrera Javier joint author Ruiz-Ferrer Virgina joint author Toro Francisco J. Del joint author Llave César joint author Voinnet Olivier joint author Elena Santiago F. joint author PLoS ONE Lawrence, KS, USA : Public Library of Science 7 (7), p. e40526 1932-6203 http://hdl.handle.net/20.500.11850/58663 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/58663 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Rodrigo Guillermo joint author ETHRESEARCH 700 1- Carrera Javier joint author ETHRESEARCH 700 1- Ruiz-Ferrer Virgina joint author ETHRESEARCH 700 1- Toro Francisco J. Del joint author ETHRESEARCH 700 1- Llave César joint author ETHRESEARCH 700 1- Voinnet Olivier joint author ETHRESEARCH 700 1- Elena Santiago F. joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, KS, USA : Public Library of Science 7 (7), p. e40526 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access