naa a22 4500 528786911 20180924065502.0 cr unu---uuuuu 180924e20121101xx s 000 0 eng 10.3929/ethz-b-000059756 doi 10.1371/journal.pone.0048271 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/59756 Co-Consumption of Methanol and Succinate by Methylobacterium extorquens AM1 [Elektronische Daten] [Rémi Peyraud, Patrick Kiefer, Philipp Christen, Jean-Charles Portais, Julia A. Vorholt] PLoS ONE Open access ethresearch Methylobacterium extorquens AM1 is a facultative methylotrophic Alphaproteobacterium and has been subject to intense study under pure methylotrophic as well as pure heterotrophic growth conditions in the past. Here, we investigated the metabolism of M. extorquens AM1 under mixed substrate conditions, i.e., in the presence of methanol plus succinate. We found that both substrates were co-consumed, and the carbon conversion was two-thirds from succinate and one-third from methanol relative to mol carbon. 13C-methanol labeling and liquid chromatography mass spectrometry analyses revealed the different fates of the carbon from the two substrates. Methanol was primarily oxidized to CO2 for energy generation. However, a portion of the methanol entered biosynthetic reactions via reactions specific to the one-carbon carrier tetrahydrofolate. In contrast, succinate was primarily used to provide precursor metabolites for bulk biomass production. This work opens new perspectives on the role of methylotrophy when substrates are simultaneously available, a situation prevailing under environmental conditions. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Peyraud Rémi joint author Kiefer Patrick joint author Christen Philipp joint author Portais Jean-Charles joint author Vorholt Julia A. joint author PLoS ONE Lawrence, KS, USA : Public Library of Science 7 (11), p. e48271 1932-6203 http://hdl.handle.net/20.500.11850/59756 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/59756 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Peyraud Rémi joint author ETHRESEARCH 700 1- Kiefer Patrick joint author ETHRESEARCH 700 1- Christen Philipp joint author ETHRESEARCH 700 1- Portais Jean-Charles joint author ETHRESEARCH 700 1- Vorholt Julia A. joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, KS, USA : Public Library of Science 7 (11), p. e48271 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access