naa a22 4500 528787004 20180924065502.0 cr unu---uuuuu 180924e20110630xx s 000 0 eng 10.3929/ethz-b-000038909 doi 10.1371/journal.pone.0021500 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/38909 Eugster Marcel R. Rapid Analysis of Listeria monocytogenes Cell Wall Teichoic Acid Carbohydrates by ESI-MS/MS [Elektronische Daten] [Marcel R. Eugster, Martin J. Loessner] PLoS ONE Open access ethresearch We report the application of electrospray ionization (ESI) mass spectrometry for compositional characterization of wall teichoic acids (WTA), a major component of Gram-positive bacterial cell walls. Tandem mass spectrometry (ESI-MS/MS) of purified and chemically hydrolyzed monomeric WTA components provided sufficient information to identify WTA monomers and their specific carbohydrate constituents. A lithium matrix was used for ionization of uncharged WTA monomers, and successfully applied to analyze the WTA molecules of four Listeria strains differing in carbohydrate substitution on a conserved polyribitol-phosphate backbone structure. Carbohydrate residues such as N-acetylglucosamine or rhamnose linked to the WTA could directly be identified by ESI-MS/MS, circumventing the need for quantitative analysis by gas chromatography. The presence of a terminal N-acetylglucosamine residue tethered to the ribitol was confirmed using fluorescently labeled wheat-germ agglutinin. In conclusion, the mass spectrometry method described here will greatly facilitate compositional analysis and characterization of teichoic acids and similar macromolecules from diverse bacterial species, and represents a significant advance in the identification of serovar-specific carbohydrates and sugar molecules on bacteria. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Loessner Martin J. joint author PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (6), p. e21500 1932-6203 http://hdl.handle.net/20.500.11850/38909 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/38909 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 100 1- Eugster Marcel R. ETHRESEARCH 700 1- Loessner Martin J. joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (6), p. e21500 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access