naa a22 4500 52878739X 20180924065503.0 cr unu---uuuuu 180924s2018 xx s 000 0 eng 10.3929/ethz-b-000289836 doi 10.1038/s41522-018-0064-3 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/289836 3D bacterial cellulose biofilms formed by foam templating [Elektronische Daten] [Patrick A. Rühs, Flavian Storz, Yuly A. López Gómez, Matthias Haug, Peter Fischer] Open access ethresearch Bacterial cellulose is a remarkable fibrous structural component of biofilms, as it forms a mechanically strong hydrogel with high water adsorption capabilities. Additionally, bacterial cellulose is biocompatible and therefore of potential interest for skin regeneration and wound healing applications. However, bacterial cellulose produced through conventional production processes at water-air interfaces lack macroporosity control, which is crucial for regenerative tissue applications. Here we demonstrate a straightforward and efficient approach to form a macroporous bacterial cellulose foam by foaming a mannitol-based media with a bacterial suspension of Gluconoacetobacter xylinus. The bacterial suspension foam is stabilized with Cremodan as a surfactant and viscosified with Xanthan preventing water drainage. Further foam stabilization occurs through cellulose formation across the foam network. As bacterial cellulose formation is influenced by the viscosity of the growth media, we fine-tuned the concentration of Xanthan to allow for bacterial cellulose formation while avoiding water drainage caused by gravity. With this simple approach, we were able to design 3D bacterial cellulose foams without any additional processing steps. We argue that this templating approach can further be used to design foamy biofilms for biotechnological approaches, increasing the surface area and therefore the yield by improving the exchange of nutrients and metabolic products. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Rühs Patrick A. joint author Storz Flavian joint author López Gómez Yuly A. joint author Haug Matthias joint author Fischer Peter joint author npj Biofilms and Microbiomes London : Nature Publishing Group 4 (1), p. 21 http://hdl.handle.net/20.500.11850/289836 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/289836 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Rühs Patrick A. joint author ETHRESEARCH 700 1- Storz Flavian joint author ETHRESEARCH 700 1- López Gómez Yuly A. joint author ETHRESEARCH 700 1- Haug Matthias joint author ETHRESEARCH 700 1- Fischer Peter joint author ETHRESEARCH 773 0- npj Biofilms and Microbiomes London : Nature Publishing Group 4 (1), p. 21 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access