naa a22 4500 52878806X CHVBK 20180924072553.0 cr unu---uuuuu 180924e201610 xx s 000 0 eng 10.1016/j.jacbts.2016.06.008 doi (SERVAL)BIB_A6AFB5D6ECD9 29707678 pmid Cardiomyocyte Lineage Specification in Adult Human Cardiac Precursor Cells Via Modulation of Enhancer-Associated Long Noncoding RNA Expression [Elektronische Daten] [I. Plaisance, S. Perruchoud, M. Fernandez-Tenorio, C. Gonzales, S. Ounzain, P. Ruchat, M. Nemir, E. Niggli, T. Pedrazzini] The mechanisms controlling differentiation in adult cardiac precursor cells (CPCs) are still largely unknown. In this study, CPCs isolated from the human heart were found to produce predominantly smooth muscle cells but could be redirected to the cardiomyocyte fate by transient activation followed by inhibition of NOTCH signaling. NOTCH inhibition repressed &lt;i&gt;MIR-143/145&lt;/i&gt; expression, and blocked smooth muscle differentiation. Expression of the microRNAs is under control of &lt;i&gt;CARMEN&lt;/i&gt; , a long noncoding RNA associated with an enhancer located in the &lt;i&gt;MIR-143/145&lt;/i&gt; locus and target of NOTCH signaling. The &lt;i&gt;CARMEN&lt;/i&gt; / &lt;i&gt;MIR-145/143&lt;/i&gt; axis represents, therefore, a promising target to favor production of cardiomyocytes in cell replacement therapies. Plaisance I. aut Perruchoud S. aut Fernandez-Tenorio M. aut Gonzales C. aut Ounzain S. aut Ruchat P. aut Nemir M. aut Niggli E. aut Pedrazzini T. aut JACC. Basic to translational science 1/6(2016-10), 472-493 1:6<472-493 2016 1 1 article serval SERVAL 700 1- Plaisance I. aut SERVAL 700 1- Perruchoud S. aut SERVAL 700 1- Fernandez-Tenorio M. aut SERVAL 700 1- Gonzales C. aut SERVAL 700 1- Ounzain S. aut SERVAL 700 1- Ruchat P. aut SERVAL 700 1- Nemir M. aut SERVAL 700 1- Niggli E. aut SERVAL 700 1- Pedrazzini T. aut SERVAL 773 0- JACC. Basic to translational science 1/6(2016-10), 472-493 1:6<472-493 2016 1 SWISSBIB 440142989 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article