caa a22 4500 52879244X 20200902091541.0 cr unu---uuuuu 180924s2015 xx s 000 0 eng 10.3929/ethz-b-000104625 doi 10.1038/ncomms9292 doi (BORIS)oai:boris.unibe.ch:77343 (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/104625 610 ddc The outer mucus layer hosts a distinct intestinal microbial niche [Elektronische Daten] [Hai Li, Julien Periclis Jean Limenitakis, Tobias Fuhrer, Markus Geuking, Melissa A Lawson, Madeleine Wyss, Sandrine Brugiroux, Irene Keller, Jamie A Macpherson, Sandra Rupp, Bettina Stolp, Jens Volker Stein, Bärbel Stecher, Uwe Sauer, Kathleen McCoy, Andrew Macpherson] Nat Commun publishedVersion openAccess eu-repo The overall composition of the mammalian intestinal microbiota varies between individuals: within each individual there are differences along the length of the intestinal tract related to host nutrition, intestinal motility and secretions. Mucus is a highly regenerative protective lubricant glycoprotein sheet secreted by host intestinal goblet cells; the inner mucus layer is nearly sterile. Here we show that the outer mucus of the large intestine forms a unique microbial niche with distinct communities, including bacteria without specialized mucolytic capability. Bacterial species present in the mucus show differential proliferation and resource utilization compared with the same species in the intestinal lumen, with high recovery of bioavailable iron and consumption of epithelial-derived carbon sources according to their genome-encoded metabolic repertoire. Functional competition for existence in this intimate layer is likely to be a major determinant of microbiota composition and microbial molecular exchange with the host. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Li Hai joint author Limenitakis Julien Periclis Jean joint author Fuhrer Tobias joint author Geuking Markus joint author Lawson Melissa A. joint author Wyss Madeleine joint author Brugiroux Sandrine joint author Keller Irene joint author Macpherson Jamie A. joint author Rupp Sandra joint author Stolp Bettina joint author Stein Jens Volker joint author Stecher Bärbel joint author Sauer Uwe joint author McCoy Kathleen joint author Macpherson Andrew joint author Nature communications 6, p. 8292 Onlinezugriff via WWW text/html Li, Hai; Limenitakis, Julien Periclis Jean; Fuhrer, Tobias; Geuking, Markus; Lawson, Melissa A; Wyss, Madeleine; Brugiroux, Sandrine; Keller, Irene; Macpherson, Jamie A; Rupp, Sandra; Stolp, Bettina; Stein, Jens Volker; Stecher, Bärbel; Sauer, Uwe; McCoy, Kathleen; Macpherson, Andrew (2015). The outer mucus layer hosts a distinct intestinal microbial niche. Nature communications, 6, p. 8292. Nature Publishing Group 10.1038/ncomms9292 <http://dx.doi.org/10.1038/ncomms9292> BORIS BK010053 XK010053 XK010000 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS BORIS BORIS info:eu-repo/semantics/article openAccess ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access BORIS 856 42 Onlinezugriff via WWW text/html Li, Hai; Limenitakis, Julien Periclis Jean; Fuhrer, Tobias; Geuking, Markus; Lawson, Melissa A; Wyss, Madeleine; Brugiroux, Sandrine; Keller, Irene; Macpherson, Jamie A; Rupp, Sandra; Stolp, Bettina; Stein, Jens Volker; Stecher, Bärbel; Sauer, Uwe; McCoy, Kathleen; Macpherson, Andrew (2015). The outer mucus layer hosts a distinct intestinal microbial niche. Nature communications, 6, p. 8292. Nature Publishing Group 10.1038/ncomms9292 <http://dx.doi.org/10.1038/ncomms9292> BORIS 700 1- Li Hai joint author BORIS 700 1- Limenitakis Julien Periclis Jean joint author BORIS 700 1- Fuhrer Tobias joint author BORIS 700 1- Geuking Markus joint author BORIS 700 1- Lawson Melissa A. joint author BORIS 700 1- Wyss Madeleine joint author BORIS 700 1- Brugiroux Sandrine joint author BORIS 700 1- Keller Irene joint author BORIS 700 1- Macpherson Jamie A. joint author BORIS 700 1- Rupp Sandra joint author BORIS 700 1- Stolp Bettina joint author BORIS 700 1- Stein Jens Volker joint author BORIS 700 1- Stecher Bärbel joint author BORIS 700 1- Sauer Uwe joint author BORIS 700 1- McCoy Kathleen joint author BORIS 700 1- Macpherson Andrew joint author BORIS 773 0- Nature communications 6, p. 8292 ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/104625 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Li Hai joint author ETHRESEARCH 700 1- Limenitakis Julien P. joint author ETHRESEARCH 700 1- Fuhrer Tobias joint author ETHRESEARCH 700 1- Geuking Markus B. joint author ETHRESEARCH 700 1- Lawson Melissa A. joint author ETHRESEARCH 700 1- Wyss Madeleine joint author ETHRESEARCH 700 1- Brugiroux Sandrine joint author ETHRESEARCH 700 1- Keller Irene joint author ETHRESEARCH 700 1- Macpherson Jamie A. joint author ETHRESEARCH 700 1- Rupp Sandra joint author ETHRESEARCH 700 1- Stolp Bettina joint author ETHRESEARCH 700 1- Stein Jens V. joint author ETHRESEARCH 700 1- Stecher Bärbel joint author ETHRESEARCH 700 1- Sauer Uwe; id_orcid 0000-0002-5923-0770 joint author ETHRESEARCH 700 1- McCoy Kathy D. joint author ETHRESEARCH 700 1- Macpherson Andrew J. joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 6, p. 8292 2041-1723