Proteomic profiling and analytical chemistry

the crossroads

Verfasser / Beitragende:
edited by P. Ciborowski, University of Nebraska Medical Center, Omaha, NE, United States, J. Silberring, AGH University of Science and Technology, Krakow, Poland; Polish Academy of Sciences, Zabrze, Poland
Ort, Verlag, Jahr:
Amsterdam, Boston : Elsevier, 2016
Beschreibung:
1 online resource (xvi, 282 pages) : illustrations
Format:
Buch (online)
Ausgabe:
Second edition.
Online Zugang:
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245 0 0 |a Proteomic profiling and analytical chemistry  |b the crossroads  |c edited by P. Ciborowski, University of Nebraska Medical Center, Omaha, NE, United States, J. Silberring, AGH University of Science and Technology, Krakow, Poland; Polish Academy of Sciences, Zabrze, Poland 
250 |a Second edition. 
264 1 |a Amsterdam  |a Boston  |b Elsevier  |c 2016 
300 |a 1 online resource (xvi, 282 pages)  |b illustrations 
336 |a text  |b txt  |2 rdacontent/eng 
337 |a computer  |b c  |2 rdamedia/eng 
338 |a online resource  |b cr  |2 rdacarrier/eng 
504 |a Includes bibliographical references and index. 
506 |a Lizenzbedingungen können den Zugang einschränken. License restrictions may limit access. 
520 |a Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.-- 
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