Six domesticated PiggyBac transposases together carry out programmed DNA elimination in
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| 245 | 0 | 0 | |a Six domesticated PiggyBac transposases together carry out programmed DNA elimination in |h [Elektronische Daten] |c [Julien Bischerour, Simran Bhullar, Cyril Denby Wilkes, Vinciane Régnier, Nathalie Mathy, Emeline Dubois, Aditi Singh, Estienne Carl Swart, Olivier Arnaiz, Linda Sperling, Mariusz Nowacki, Mireille Bétermier] |
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| 520 | 3 | |a The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in constitute a rare example of a biological process jointly managed by six distinct domesticated transposases. | |
| 700 | 1 | |a Bischerour |D Julien |e joint author | |
| 700 | 1 | |a Bhullar |D Simran |e joint author | |
| 700 | 1 | |a Denby Wilkes |D Cyril |e joint author | |
| 700 | 1 | |a Régnier |D Vinciane |e joint author | |
| 700 | 1 | |a Mathy |D Nathalie |e joint author | |
| 700 | 1 | |a Dubois |D Emeline |e joint author | |
| 700 | 1 | |a Singh |D Aditi |e joint author | |
| 700 | 1 | |a Swart |D Estienne Carl |e joint author | |
| 700 | 1 | |a Arnaiz |D Olivier |e joint author | |
| 700 | 1 | |a Sperling |D Linda |e joint author | |
| 700 | 1 | |a Nowacki |D Mariusz |e joint author | |
| 700 | 1 | |a Bétermier |D Mireille |e joint author | |
| 773 | 0 | |t eLife | |
| 856 | 4 | 2 |
|z Onlinezugriff via WWW
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|u Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927 |
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| 908 | |D 1 |a info:eu-repo/semantics/article |2 boris | ||
| 909 | 7 | |a PeerReviewed |2 boris peerstatus | |
| 949 | |B BORIS |F BORIS |b BORIS |j info:eu-repo/semantics/article |c openAccess | ||
| 950 |
|B BORIS
|P 856
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|z Onlinezugriff via WWW
|q text/html
|u Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927 |
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| 950 | |B BORIS |P 700 |E 1- |a Bischerour |D Julien |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Bhullar |D Simran |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Denby Wilkes |D Cyril |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Régnier |D Vinciane |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Mathy |D Nathalie |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Dubois |D Emeline |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Singh |D Aditi |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Swart |D Estienne Carl |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Arnaiz |D Olivier |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Sperling |D Linda |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Nowacki |D Mariusz |e joint author | ||
| 950 | |B BORIS |P 700 |E 1- |a Bétermier |D Mireille |e joint author | ||
| 950 | |B BORIS |P 773 |E 0- |t eLife | ||