caa a22 4500 529013487 20201130031323.0 cr unu---uuuuu 180927e20180918xx s 000 0 eng (BORIS)oai:boris.unibe.ch:120124 570 ddc Six domesticated PiggyBac transposases together carry out programmed DNA elimination in [Elektronische Daten] [Julien Bischerour, Simran Bhullar, Cyril Denby Wilkes, Vinciane Régnier, Nathalie Mathy, Emeline Dubois, Aditi Singh, Estienne Carl Swart, Olivier Arnaiz, Linda Sperling, Mariusz Nowacki, Mireille Bétermier] publishedVersion openAccess eu-repo The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in constitute a rare example of a biological process jointly managed by six distinct domesticated transposases. Bischerour Julien joint author Bhullar Simran joint author Denby Wilkes Cyril joint author Régnier Vinciane joint author Mathy Nathalie joint author Dubois Emeline joint author Singh Aditi joint author Swart Estienne Carl joint author Arnaiz Olivier joint author Sperling Linda joint author Nowacki Mariusz joint author Bétermier Mireille joint author eLife Onlinezugriff via WWW text/html Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927 <http://dx.doi.org/10.7554/eLife.37927> BORIS BK010053 XK010053 XK010000 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS BORIS BORIS info:eu-repo/semantics/article openAccess BORIS 856 42 Onlinezugriff via WWW text/html Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927 <http://dx.doi.org/10.7554/eLife.37927> BORIS 700 1- Bischerour Julien joint author BORIS 700 1- Bhullar Simran joint author BORIS 700 1- Denby Wilkes Cyril joint author BORIS 700 1- Régnier Vinciane joint author BORIS 700 1- Mathy Nathalie joint author BORIS 700 1- Dubois Emeline joint author BORIS 700 1- Singh Aditi joint author BORIS 700 1- Swart Estienne Carl joint author BORIS 700 1- Arnaiz Olivier joint author BORIS 700 1- Sperling Linda joint author BORIS 700 1- Nowacki Mariusz joint author BORIS 700 1- Bétermier Mireille joint author BORIS 773 0- eLife