caa a22 4500 530060035 20181106030140.0 cr unu---uuuuu 181025s2018 xx s 000 0 eng 2398-502X 10.12688/wellcomeopenres.14645.3 doi (EDOC)oai:edoc.unibas.ch:66262 30320226 pmid Probing Plasmodium falciparum sexual commitment at the single-cell level [Elektronische Daten] [Nicolas M. B. Brancucci, Mariana De Niz, Timothy J. Straub, Deepali Ravel, Lauriane Sollelis, Bruce W. Birren, Till S. Voss, Daniel E. Neafsey, Matthias Marti] Background:; Malaria parasites go through major transitions during their complex life cycle, yet the underlying differentiation pathways remain obscure. Here we apply single cell transcriptomics to unravel the program inducing sexual differentiation in; Plasmodium falciparum; . Parasites have to make this essential life-cycle decision in preparation for human-to-mosquito transmission.; Methods:; By combining transcriptional profiling with quantitative imaging and genetics, we defined a transcriptional signature in sexually committed cells.; Results:; We found this transcriptional signature to be distinct from general changes in parasite metabolism that can be observed in response to commitment-inducing conditions.; Conclusions:; This proof-of-concept study provides a template to capture transcriptional diversity in parasite populations containing complex mixtures of different life-cycle stages and developmental programs, with important implications for our understanding of parasite biology and the ongoing malaria elimination campaign. Brancucci Nicolas M. B. joint author De Niz Mariana joint author Straub Timothy J. joint author Ravel Deepali joint author Sollelis Lauriane joint author Birren Bruce W. joint author Voss Till S. joint author Neafsey Daniel E. joint author Marti Matthias joint author Wellcome Open Research 3, p. 70 2398-502X https://edoc.unibas.ch/66262/1/20181023092541_5bceccf5e686d.pdf text/html WWW-Backlink auf das Repository 1 Article edoc PeerReviewed edoc peerstatus EDOC 856 40 https://edoc.unibas.ch/66262/1/20181023092541_5bceccf5e686d.pdf text/html WWW-Backlink auf das Repository EDOC 700 1- Brancucci Nicolas M. B. joint author EDOC 700 1- De Niz Mariana joint author EDOC 700 1- Straub Timothy J. joint author EDOC 700 1- Ravel Deepali joint author EDOC 700 1- Sollelis Lauriane joint author EDOC 700 1- Birren Bruce W. joint author EDOC 700 1- Voss Till S. joint author EDOC 700 1- Neafsey Daniel E. joint author EDOC 700 1- Marti Matthias joint author EDOC 773 0- Wellcome Open Research 3, p. 70 2398-502X BK010053 XK010053 XK010000 EDOC EDOC EDOC Article