naa a22 4500 530098199 20181026074612.0 cr unu---uuuuu 181026e20140926xx s 000 0 eng 10.3929/ethz-b-000297623 doi 10.1371/journal.pone.0106628 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/297623 A Window of Opportunity to Control the Bacterial Pathogen Pseudomonas aeruginosa Combining Antibiotics and Phages [Elektronische Daten] [Clara Torres-Barceló, Flor I. Arias-Sánchez, Marie; id_orcid 0000-0003-4081-6664 Vasse, Johan Ramsayer, Oliver Kaltz, Michael E. Hochberg] PLoS ONE Open access ethresearch The evolution of antibiotic resistance in bacteria is a global concern and the use of bacteriophages alone or in combined therapies is attracting increasing attention as an alternative. Evolutionary theory predicts that the probability of bacterial resistance to both phages and antibiotics will be lower than to either separately, due for example to fitness costs or to trade-offs between phage resistance mechanisms and bacterial growth. In this study, we assess the population impacts of either individual or combined treatments of a bacteriophage and streptomycin on the nosocomial pathogen Pseudomonas aeruginosa. We show that combining phage and antibiotics substantially increases bacterial control compared to either separately, and that there is a specific time delay in antibiotic introduction independent of antibiotic dose, that minimizes both bacterial density and resistance to either antibiotics or phage. These results have implications for optimal combined therapeutic approaches. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Torres-Barceló Clara joint author Arias-Sánchez Flor I. joint author Vasse Marie; id_orcid 0000-0003-4081-6664 joint author Ramsayer Johan joint author Kaltz Oliver joint author Hochberg Michael E. joint author PLoS ONE San Francisco, CA : Public Library of Science 9 (9), p. e106628 1932-6203 http://hdl.handle.net/20.500.11850/297623 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/297623 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Torres-Barceló Clara joint author ETHRESEARCH 700 1- Arias-Sánchez Flor I. joint author ETHRESEARCH 700 1- Vasse Marie; id_orcid 0000-0003-4081-6664 joint author ETHRESEARCH 700 1- Ramsayer Johan joint author ETHRESEARCH 700 1- Kaltz Oliver joint author ETHRESEARCH 700 1- Hochberg Michael E. joint author ETHRESEARCH 773 0- PLoS ONE San Francisco, CA : Public Library of Science 9 (9), p. e106628 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access