caa a22 4500 53009830X 20181217030326.0 cr unu---uuuuu 181026e20160908xx s 000 0 eng 10.3929/ethz-b-000120305 doi 10.1038/srep32824 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/120305 Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones [Elektronische Daten] [Jehangir Cama, Michael Schaich, Kareem Al Nahas, Silvia Hernández-Ainsa, Stefano Pagliara, Ulrich F. Keyser] Sci Rep Open access ethresearch Quantifying drug permeability across lipid membranes is crucial for drug development. In addition, reduced membrane permeability is a leading cause of antibiotic resistance in bacteria, and hence there is a need for new technologies that can quantify antibiotic transport across biological membranes. We recently developed an optofluidic assay that directly determines the permeability coefficient of autofluorescent drug molecules across lipid membranes. Using ultraviolet fluorescence microscopy, we directly track drug accumulation in giant lipid vesicles as they traverse a microfluidic device while exposed to the drug. Importantly, our measurement does not require the knowledge of the octanol partition coefficient of the drug - we directly determine the permeability coefficient for the specific drug-lipid system. In this work, we report measurements on a range of fluoroquinolone antibiotics and find that their pH dependent lipid permeability can span over two orders of magnitude. We describe various technical improvements for our assay, and provide a new graphical user interface for data analysis to make the technology easier to use for the wider community. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Permeation and transport ethresearch Drug delivery ethresearch Cama Jehangir joint author Schaich Michael joint author Al Nahas Kareem joint author Hernández-Ainsa Silvia joint author Pagliara Stefano joint author Keyser Ulrich F. joint author Scientific Reports London : Nature Publishing Group 6, p. 32824 2045-2322 http://hdl.handle.net/20.500.11850/120305 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/120305 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Cama Jehangir joint author ETHRESEARCH 700 1- Schaich Michael joint author ETHRESEARCH 700 1- Al Nahas Kareem joint author ETHRESEARCH 700 1- Hernández-Ainsa Silvia joint author ETHRESEARCH 700 1- Pagliara Stefano joint author ETHRESEARCH 700 1- Keyser Ulrich F. joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 6, p. 32824 2045-2322 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access