caa a22 4500 530098741 20200103030211.0 cr unu---uuuuu 181026e20151207xx s 000 0 eng 10.3929/ethz-b-000110814 doi 10.1038/srep17609 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/110814 Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabrea: Striking genomic and morphological features of novel Endozoicomonas sp [Elektronische Daten] [Pantelis Katharios, Helena M.B. Seth-Smith, Alexander Fehr, José M. Mateos, Weihong Qi, Denis Richter, Lisbeth Nufer, Maricruz Ruetten, Maricruz Guevara Soto, Urs Ziegler, Nicholas R. Thomson, Ralph Schlapbach, Lloyd Vaughan] Open access ethresearch Aquaculture is a burgeoning industry, requiring diversification into new farmed species, which are often at risk from infectious disease. We used a mesocosm technique to investigate the susceptibility of sharpsnout seabream (Diplodus puntazzo) larvae to potential environmental pathogens in seawater compared to control borehole water. Fish exposed to seawater succumbed to epitheliocystis from 21 days post hatching, causing mortality in a quarter of the hosts. The pathogen responsible was not chlamydial, as is often found in epitheliocystis, but a novel species of the γ-proteobacterial genus Endozoicomonas. Detailed characterisation of this pathogen within the infectious lesions using high resolution fluorescent and electron microscopy showed densely packed rod shaped bacteria. A draft genome sequence of this uncultured bacterium was obtained from preserved material. Comparison with the genome of the Endozoicomonas elysicola type strain shows that the genome of Ca. Endozoicomonas cretensis is undergoing decay through loss of functional genes and insertion sequence expansion, often indicative of adaptation to a new niche or restriction to an alternative lifestyle. These results demonstrate the advantage of mesocosm studies for investigating the effect of environmental bacteria on susceptible hosts and provide an important insight into the genome dynamics of a novel fish pathogen. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Genome ethresearch Imaging ethresearch Water microbiology ethresearch Katharios Pantelis joint author Seth-Smith Helena M.B. joint author Fehr Alexander joint author Mateos José M. joint author Qi Weihong joint author Richter Denis joint author Nufer Lisbeth joint author Ruetten Maricruz joint author Guevara Soto Maricruz joint author Ziegler Urs joint author Thomson Nicholas R. joint author Schlapbach Ralph joint author Vaughan Lloyd joint author Scientific Reports London : Nature Publishing Group 5, p. 17609 2045-2322 http://hdl.handle.net/20.500.11850/110814 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/110814 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Katharios Pantelis joint author ETHRESEARCH 700 1- Seth-Smith Helena M.B. joint author ETHRESEARCH 700 1- Fehr Alexander joint author ETHRESEARCH 700 1- Mateos José M. joint author ETHRESEARCH 700 1- Qi Weihong joint author ETHRESEARCH 700 1- Richter Denis joint author ETHRESEARCH 700 1- Nufer Lisbeth joint author ETHRESEARCH 700 1- Ruetten Maricruz joint author ETHRESEARCH 700 1- Guevara Soto Maricruz joint author ETHRESEARCH 700 1- Ziegler Urs joint author ETHRESEARCH 700 1- Thomson Nicholas R. joint author ETHRESEARCH 700 1- Schlapbach Ralph joint author ETHRESEARCH 700 1- Vaughan Lloyd joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 5, p. 17609 2045-2322 SWISSBIB 354607405 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access