caa a22 4500 53009889X 20181217030327.0 cr unu---uuuuu 181026e20160525xx s 000 0 eng 10.3929/ethz-b-000117252 doi 10.1038/srep26702 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/117252 Molecular Architecture of Yeast Chromatin Assembly Factor 1 [Elektronische Daten] [Daegeun Kim, Dheva Setiaputra, Taeyang Jung, Jaehee Chung, Alexander Leitner, Jungmin Yoon, Ruedi Aebersold, Hans Hebert, Calvin K. Yip, Ji-Joon Song] Sci Rep Open access ethresearch Chromatin Assembly Complex 1 (CAF-1) is a major histone chaperone involved in deposition of histone H3 and H4 into nucleosome. CAF-1 is composed of three subunits; p150, p60 and p48 for human and Cac1, Cac2 and Cac3 for yeast. Despite of its central role in chromatin formation, structural features of the full CAF-1 in complex with histones and other chaperones have not been well characterized. Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that Cac1, the largest subunit of yCAF-1, might serve as a major histone binding platform linking Cac2 and Cac3. In addition, EM analysis showed that yCAF-1 adopts a bilobal shape and Cac1 connecting Cac2 and Cac3 to generate a platform for binding histones. This study provides the first structural glimpse of the full CAF-1 complex and a structural framework to understand histone chaperoning processes. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Epigenetics ethresearch Structural biology ethresearch Kim Daegeun joint author Setiaputra Dheva joint author Jung Taeyang joint author Chung Jaehee joint author Leitner Alexander joint author Yoon Jungmin joint author Aebersold Ruedi joint author Hebert Hans joint author Yip Calvin K. joint author Song Ji-Joon joint author Scientific Reports London : Nature Publishing Group 6, p. 26702 2045-2322 http://hdl.handle.net/20.500.11850/117252 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/117252 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Kim Daegeun joint author ETHRESEARCH 700 1- Setiaputra Dheva joint author ETHRESEARCH 700 1- Jung Taeyang joint author ETHRESEARCH 700 1- Chung Jaehee joint author ETHRESEARCH 700 1- Leitner Alexander joint author ETHRESEARCH 700 1- Yoon Jungmin joint author ETHRESEARCH 700 1- Aebersold Ruedi joint author ETHRESEARCH 700 1- Hebert Hans joint author ETHRESEARCH 700 1- Yip Calvin K. joint author ETHRESEARCH 700 1- Song Ji-Joon joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 6, p. 26702 2045-2322 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access