naa a22 4500 530185261 CHVBK 20181026074444.0 cr unu---uuuuu 181026e201809 xx s 000 0 eng 10.1038/s41596-018-0030-9 doi (SERVAL)BIB_94B9EA9825A4 30190556 pmid 000444448400006 isiid Configuration of electrical spinal cord stimulation through real-time processing of gait kinematics [Elektronische Daten] [M. Capogrosso, F.B. Wagner, J. Gandar, E.M. Moraud, N. Wenger, T. Milekovic, P. Shkorbatova, N. Pavlova, P. Musienko, E. Bezard, J. Bloch, G. Courtine] Epidural electrical stimulation (EES) of the spinal cord and real-time processing of gait kinematics are powerful methods for the study of locomotion and the improvement of motor control after injury or in neurological disorders. Here, we describe equipment and surgical procedures that can be used to acquire chronic electromyographic (EMG) recordings from leg muscles and to implant targeted spinal cord stimulation systems that remain stable up to several months after implantation in rats and nonhuman primates. We also detail how to exploit these implants to configure electrical spinal cord stimulation policies that allow control over the degree of extension and flexion of each leg during locomotion. This protocol uses real-time processing of gait kinematics and locomotor performance, and can be configured within a few days. Once configured, stimulation bursts are delivered over specific spinal cord locations with precise timing that reproduces the natural spatiotemporal activation of motoneurons during locomotion. These protocols can also be easily adapted for the safe implantation of systems in the vicinity of the spinal cord and to conduct experiments involving real-time movement feedback and closed-loop controllers. Capogrosso M. aut Wagner F.B. aut Gandar J. aut Moraud E.M. aut Wenger N. aut Milekovic T. aut Shkorbatova P. aut Pavlova N. aut Musienko P. aut Bezard E. aut Bloch J. aut Courtine G. aut Nature protocols 13/9(2018-09), 2031-2061 13:9<2031-2061 2018 13 1 article serval SERVAL 700 1- Capogrosso M. aut SERVAL 700 1- Wagner F.B. aut SERVAL 700 1- Gandar J. aut SERVAL 700 1- Moraud E.M. aut SERVAL 700 1- Wenger N. aut SERVAL 700 1- Milekovic T. aut SERVAL 700 1- Shkorbatova P. aut SERVAL 700 1- Pavlova N. aut SERVAL 700 1- Musienko P. aut SERVAL 700 1- Bezard E. aut SERVAL 700 1- Bloch J. aut SERVAL 700 1- Courtine G. aut SERVAL 773 0- Nature protocols 13/9(2018-09), 2031-2061 13:9<2031-2061 2018 13 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article