naa a22 4500 530185512 CHVBK 20181026074446.0 cr unu---uuuuu 181026e201810 xx s 000 0 eng 10.1177/0271678X17734101 doi (SERVAL)BIB_5C44786165AA 29047296 pmid 000446200600004 isiid Invivo 13C MRS in the mouse brain at 14.1 Tesla and metabolic flux quantification under infusion of [Elektronische Daten] [M. Lai, B. Lanz, C. Poitry-Yamate, J.F. Romero, C.M. Berset, C. Cudalbu, R. Gruetter] 1,6-13C2 Invivo 13 C magnetic resonance spectroscopy (MRS) enables the investigation of cerebral metabolic compartmentation while, e.g. infusing 13 C-labeled glucose. Metabolic flux analysis of 13 C turnover previously yielded quantitative information of glutamate and glutamine metabolism in humans and rats, while the application to invivo mouse brain remains exceedingly challenging. In the present study, 13 C direct detection at 14.1 T provided highly resolved invivo spectra of the mouse brain while infusing [1,6- 13 C 2 ]glucose for up to 5 h. 13 C incorporation to glutamate and glutamine C4, C3, and C2 and aspartate C3 were detected dynamically and fitted to a two-compartment model: flux estimation of neuron-glial metabolism included tricarboxylic acid cycle (TCA) flux in astrocytes (V g = 0.16 ± 0.03 µmol/g/min) and neurons (V TCA n = 0.56 ± 0.03 µmol/g/min), pyruvate carboxylase activity (V PC = 0.041 ± 0.003 µmol/g/min) and neurotransmission rate (V NT = 0.084 ± 0.008 µmol/g/min), resulting in a cerebral metabolic rate of glucose (CMR glc ) of 0.38 ± 0.02 µmol/g/min, in excellent agreement with that determined with concomitant 18 F-fluorodeoxyglucose positron emission tomography ( 18 FDG PET).We conclude that modeling of neuron-glial metabolism invivo is accessible in the mouse brain from 13 C direct detection with an unprecedented spatial resolution under [1,6- 13 C 2 ]glucose infusion. Lai M. aut Lanz B. aut Poitry-Yamate C. aut Romero J.F. aut Berset C.M. aut Cudalbu C. aut Gruetter R. aut Journal of cerebral blood flow and metabolism 38/10(2018-10), 1701-1714 38:10<1701-1714 2018 38 1 article serval SERVAL 700 1- Lai M. aut SERVAL 700 1- Lanz B. aut SERVAL 700 1- Poitry-Yamate C. aut SERVAL 700 1- Romero J.F. aut SERVAL 700 1- Berset C.M. aut SERVAL 700 1- Cudalbu C. aut SERVAL 700 1- Gruetter R. aut SERVAL 773 0- Journal of cerebral blood flow and metabolism 38/10(2018-10), 1701-1714 38:10<1701-1714 2018 38 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article