caa a22 4500 530216876 CHVBK 20181123193442.0 cr unu---uuuuu 181027e20181002xx s 000 0 eng 10.1073/pnas.1807049115 doi (SERVAL)BIB_3CB652F83F01 30228123 pmid 000446078700092 isiid Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant [Elektronische Daten] [C.T. Nguyen, A. Kurenda, S. Stolz, A. Chételat, E.E. Farmer] The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In &lt;i&gt;Arabidopsis&lt;/i&gt; , wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both of these spatially separated cell types strongly attenuated leaf-to-leaf electrical signaling. &lt;i&gt;glr3.3&lt;/i&gt; mutants were also compromised in their defense against herbivores. Since wounding is known to cause increases in cytosolic calcium, we monitored electrical signals and Ca &lt;sup&gt;2+&lt;/sup&gt; transients simultaneously. This revealed that wound-induced membrane depolarizations in the wild-type preceded cytosolic Ca &lt;sup&gt;2+&lt;/sup&gt; maxima. The axial and radial distributions of calcium fluxes were differentially affected in each &lt;i&gt;glr&lt;/i&gt; mutant. Resolving a debate over which cell types are necessary for electrical signaling between leaves, we show that phloem sieve elements and xylem contact cells function together in this process. Nguyen C.T. aut Kurenda A. aut Stolz S. aut Chételat A. aut Farmer E.E. aut Proceedings of the National Academy of Sciences of the United States of America 115/40(2018-10-02), 10178-10183 115:40<10178-10183 2018 115 1 article serval SERVAL 700 1- Nguyen C.T. aut SERVAL 700 1- Kurenda A. aut SERVAL 700 1- Stolz S. aut SERVAL 700 1- Chételat A. aut SERVAL 700 1- Farmer E.E. aut SERVAL 773 0- Proceedings of the National Academy of Sciences of the United States of America 115/40(2018-10-02), 10178-10183 115:40<10178-10183 2018 115 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article