caa a22 4500 530216906 CHVBK 20190430031508.0 cr unu---uuuuu 181027e201810 xx s 000 0 eng 10.1038/s41556-018-0199-8 doi (SERVAL)BIB_63896D903CD5 30250060 pmid 000445656400012 isiid Direct induction of microtubule branching by microtubule nucleation factor SSNA1 [Elektronische Daten] [N. Basnet, H. Nedozralova, A.H. Crevenna, S. Bodakuntla, T. Schlichthaerle, M. Taschner, G. Cardone, C. Janke, R. Jungmann, M.M. Magiera, C. Biertümpfel, N. Mizuno] Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules can be directly remodelled into branched structures by the microtubule-remodelling factor SSNA1 (also known as NA14 or DIP13). The branching activity of SSNA1 relies on its ability to self-assemble into fibrils in a head-to-tail fashion. SSNA1 fibrils guide protofilaments of a microtubule to split apart to form daughter microtubules. We further found that SSNA1 localizes at axon branching sites and has a key role in neuronal development. SSNA1 mutants that abolish microtubule branching in vitro also fail to promote axon development and branching when overexpressed in neurons. We have, therefore, discovered a mechanism for microtubule branching and implicated its role in neuronal development. Basnet N. aut Nedozralova H. aut Crevenna A.H. aut Bodakuntla S. aut Schlichthaerle T. aut Taschner M. aut Cardone G. aut Janke C. aut Jungmann R. aut Magiera M.M. aut Biertümpfel C. aut Mizuno N. aut Nature cell biology 20/10(2018-10), 1172-1180 20:10<1172-1180 2018 20 1 article serval SERVAL 700 1- Basnet N. aut SERVAL 700 1- Nedozralova H. aut SERVAL 700 1- Crevenna A.H. aut SERVAL 700 1- Bodakuntla S. aut SERVAL 700 1- Schlichthaerle T. aut SERVAL 700 1- Taschner M. aut SERVAL 700 1- Cardone G. aut SERVAL 700 1- Janke C. aut SERVAL 700 1- Jungmann R. aut SERVAL 700 1- Magiera M.M. aut SERVAL 700 1- Biertümpfel C. aut SERVAL 700 1- Mizuno N. aut SERVAL 773 0- Nature cell biology 20/10(2018-10), 1172-1180 20:10<1172-1180 2018 20 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article