cam a22 2 4500 551380446 CHVBK 20190412150903.0 m d cr |n |||||||| 140810e20130813nyua s|||| 0 |eng|d 978-1-4899-4038-4 1-4899-4038-3 (Trade Paper) : USD 149.99 Retail Price (Springer) 9781489940384 (SERSOL)ssib031400088 (WaSeSS)ssib031400088 BIP US WaSeSS QH573-671 574.87/3283 20 PCR Sequencing Protocols [Elektronische Daten] New York Secaucus Humana Press Springer [Distributor] Aug. 2013 1 online resource (xi, 221 p.) ill Methods in Molecular Biology Ser. 65 Methods in Molecular Biology Ser. 65 Lizenzbedingungen können den Zugang einschränken. License restrictions may limit access. Annotation Advances in bioscience research usually arise as a result of the continu ing refinement of existing technologies. However, there are a number of occa sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac tion. This technique, first reported in 1985 by MuUis and his colleagues, pro vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase. Scholarly & Professional Humana Press Rapley Ralph Editor edt https://link.springer.com/10.1385/0896033449 Uni Basel: Volltext https://link.springer.com/10.1385/0896033449 Uni Bern: Volltext Springer Protocols E-Books von 360MarcUpdates IDSBB 490 1- Methods in Molecular Biology Ser 65 IDSBB 700 1- Rapley Ralph Editor edt IDSBB 856 40 https://link.springer.com/10.1385/0896033449 Uni Basel: Volltext IDSBB 856 40 https://link.springer.com/10.1385/0896033449 Uni Bern: Volltext BK020053 XK020053 XK020000 IDSBB A145 A145 145VT NELA1451812 IDSBB B405 B405 405VT NELB4051812