cam a22 2 4500 551381485 CHVBK 20190412151123.0 m d cr |n |||||||| 140531e20130816nyua s|||| 0 |eng|d 978-1-4899-4080-3 1-4899-4080-4 (Trade Paper) : USD 149.99 Retail Price (Springer) 9781489940803 (SERSOL)ssib031400090 (WaSeSS)ssib031400090 BIP US WaSeSS QH573-671 581.87/328 20 Plant Cell Electroporation and Electrofusion Protocols [Elektronische Daten] New York Secaucus Humana Press Springer [Distributor] Aug. 2013 1 online resource (xiv, 206 p.) ill Methods in Molecular Biology Ser. 55 Methods in Molecular Biology Ser. 55 Lizenzbedingungen können den Zugang einschränken. License restrictions may limit access. Annotation Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells including heat and chemical treatments, and microinjection electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals." Scholarly & Professional Humana Press Nickoloff Jac A. Editor edt https://link.springer.com/10.1385/0896033287 Uni Basel: Volltext https://link.springer.com/10.1385/0896033287 Uni Bern: Volltext Springer Protocols E-Books von 360MarcUpdates IDSBB 490 1- Methods in Molecular Biology Ser 55 IDSBB 700 1- Nickoloff Jac A. Editor edt IDSBB 856 40 https://link.springer.com/10.1385/0896033287 Uni Basel: Volltext IDSBB 856 40 https://link.springer.com/10.1385/0896033287 Uni Bern: Volltext SWISSBIB 139513841 BK020053 XK020053 XK020000 IDSBB A145 A145 145VT NELA1451812 IDSBB B405 B405 405VT NELB4051812