cad a22 4500 551414529 20190111035347.0 cr unu---uuuuu 181214e20190101xx s 000 0 eng 10.1007/s00383-018-4383-5 doi (ZORA)oai:www.zora.uzh.ch:159323 610 ddc 30382375 pmid Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes [Elektronische Daten] [Katarzyna Michalak-Micka, Agnes S Klar, Sophie Böttcher-Haberzeth, Ernst Reichmann, Martin Meuli, Thomas Biedermann] closedAccess eu-repo AIM OF THE STUDY The use of autologous bio-engineered dermo-epidermal skin substitutes (DESS) yields a pivotal opportunity to cover large skin defects in human patients. These skin grafts consist of both epidermal and dermal compartments necessary for robust and permanent functional wound closure. In this study, we investigated the impact of mesenchymal cells derived from different body site origins on the expression pattern of diverse markers within DESS. METHODS Human keratinocytes were obtained from interfollicular epidermis, and mesenchymal cells were isolated from foreskin, palmar skin, fat tissue, and tonsils. After expansion, epidermal cells were seeded on collagen I hydrogels containing stromal cells. These human DESS were transplanted on the back of immune-incompetent rats. After 3 weeks, transplants were excised and analyzed using immunohistology techniques. MAIN RESULTS The macroscopic appearance of skin grafts containing tonsil, fat tissue, or palmar derived mesenchymal cells, was similar to substitutes with foreskin derived dermal fibroblasts. All skin grafts had a strong membrane-localized expression of Lingo-1 in the epidermis. Additionally, we observed an intense expression of transglutaminase 5 in upper epidermal cell layers of the skin grafts confirming a proper keratinocyte differentiation. Tropoelastin was localized throughout the dermal compartments and tightly in contact with the dermo-epidermal junction suggesting an advanced maturation of all skin grafts. CONCLUSIONS Our data implicate that stromal cells derived from tonsil, fat tissue, and palmar skin can assume fibroblast functions supporting keratinocyte proliferation and differentiation. These findings indicate that distinct types of mesenchymal cells can be clinically used for skin engineering purposes. Clinic for Surgery zora Michalak-Micka Katarzyna joint author Klar Agnes S. joint author Böttcher-Haberzeth Sophie joint author Reichmann Ernst joint author Meuli Martin joint author Biedermann Thomas joint author Pediatric Surgery International 35(1):121-127 https://www.zora.uzh.ch/159323 text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/159323 text/html WWW-Backlink auf das Repository ZORA 700 1- Michalak-Micka Katarzyna joint author ZORA 700 1- Klar Agnes S. joint author ZORA 700 1- Böttcher-Haberzeth Sophie joint author ZORA 700 1- Reichmann Ernst joint author ZORA 700 1- Meuli Martin joint author ZORA 700 1- Biedermann Thomas joint author ZORA 773 0- Pediatric Surgery International 35(1):121-127 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article closedAccess