caa a22 4500 551458313 CHVBK 20190303133304.0 cr unu---uuuuu 181215e20181127xx s 000 0 eng 10.1016/j.celrep.2018.11.007 doi (SERVAL)BIB_B4439E9EC71E 30485808 pmid 000451469100009 isiid Antibiotic-Induced Cell Chaining Triggers Pneumococcal Competence by Reshaping Quorum Sensing to Autocrine-Like Signaling [Elektronische Daten] [A. Domenech, J. Slager, J.W. Veening] Streptococcus pneumoniae can acquire antibiotic resistance by activation of competence and subsequent DNA uptake. Here, we demonstrate that aztreonam (ATM) and clavulanic acid (CLA) promote competence. We show that both compounds induce cell chain formation by targeting the d,d-carboxypeptidase PBP3. In support of the hypothesis that chain formation promotes competence, we demonstrate that an autolysin mutant (ΔlytB) is hypercompetent. Since competence is initiated by the binding of a small extracellular peptide (CSP) to a membrane-anchored receptor (ComD), we wondered whether chain formation alters CSP diffusion kinetics. Indeed, ATM or CLA presence affects competence synchronization by shifting from global to local quorum sensing, as CSP is primarily retained to chained cells,rather than shared in a common pool. Importantly, autocrine-like signaling prolongs the time window in which the population is able to take up DNA. Together, these insights demonstrate the versatility of quorum sensing and highlight the importance of an accurate antibiotic prescription. Domenech A. aut Slager J. aut Veening J.W. aut Cell reports 25/9(2018-11-27), 2390-2400.e3 25:9<2390-2400.e3 2018 25 1 article serval SERVAL 700 1- Domenech A. aut SERVAL 700 1- Slager J. aut SERVAL 700 1- Veening J.W. aut SERVAL 773 0- Cell reports 25/9(2018-11-27), 2390-2400.e3 25:9<2390-2400.e3 2018 25 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article