cad a22 4500 551468084 20200417040219.0 cr unu---uuuuu 181215e20181001xx s 000 0 eng 10.5167/uzh-159392 doi 10.5005/jp-journals-10024-2400 doi (ZORA)oai:www.zora.uzh.ch:159392 610 ddc 30498169 pmid In Vitro Assessment of Cytotoxicity and Estrogenicity of Vivera® Retainers [Elektronische Daten] [Shaima R Al Naqbi, Harris Pratsinis, Dimitris Kletsas, Theodore Eliades, Athanasios E Athanasiou] openAccess eu-repo AIM To investigate the cytotoxicity and estrogenicity of Vivera® retainers by assessing their biological behavioral effects as-received from the manufacturer and after retrieved from patients. MATERIALS AND METHODS In this, in vitro investigation six sets (maxillary and mandibular) of Vivera® retainers, three as received and three retrieved after four weeks of use by patients of an orthodontic postgraduate clinic, were immersed in the normal saline solution for 14 days following different modes of sterilization. The estrogenicity assays involved two cell lines, namely the estrogen-sensitive MCF-7 and the estrogen-insensitive MDA-MB-231. Following a 6 day incubation with the solutions to be tested, at concentrations varying from 5% to 20% v/v in medium supplemented with 2% fetal calf serum devoid of endogenous estrogens, estrogenicity was assessed by cell counting; p-Estradiol was used as positive control. The statistical analysis of data was performed with two-way analysis of variance (ANOVA) with appliance and concentration as predictors. Differences were further investigated with the Tukey multiple comparison tests at the 0.05 level of significance. RESULTS No significant MCF-7 proliferation was induced by the three samples compared either to the eluents from as-received retainers or to the negative control. As expected, p-estradiol induced a potent stimulation of MCF-7 cell proliferation, while no effect was observed on MDA-MB-231 cells. CONCLUSION Under the conditions of this experiment eluents of as-received and retrieved Vivera® retainers did not seem to exhibit xenoestrogenic activity. CLINICAL SIGNIFICANCE Vivera® retainers can be used as part-time removable oral appliances following the manufacturer's instructions. Clinic for Orthodontics and Pediatric Dentistry zora Al Naqbi Shaima R. joint author Pratsinis Harris joint author Kletsas Dimitris joint author Eliades Theodore joint author Athanasiou Athanasios E. joint author Journal of Contemporary Dental Practice 19(10):1163-1168 https://www.zora.uzh.ch/id/eprint/159392/1/Al_Naqbi.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/159392/1/Al_Naqbi.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Al Naqbi Shaima R. joint author ZORA 700 1- Pratsinis Harris joint author ZORA 700 1- Kletsas Dimitris joint author ZORA 700 1- Eliades Theodore joint author ZORA 700 1- Athanasiou Athanasios E. joint author ZORA 773 0- Journal of Contemporary Dental Practice 19(10):1163-1168 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess