cad a22 4500 551468238 20200417040220.0 cr unu---uuuuu 181215e20190101xx s 000 0 eng 10.5167/uzh-159414 doi 10.1111/jcpe.13035 doi (ZORA)oai:www.zora.uzh.ch:159414 610 ddc 30372568 pmid Healing kinetics of oral soft tissue wounds treated with recombinant epidermal growth factor: Translation from a canine model [Elektronische Daten] [Heithem Ben Amara, Daniel S Thoma, Frank Schwarz, Hyun Young Song, Joseph Capetillo, Ki-Tae Koo] openAccess eu-repo OBJECTIVE To test whether or not topically administered recombinant human epidermal growth factor (rhEGF) accelerates the early healing phase of oral soft tissue wounds. METHODS One day following the creation of palatal defects (n=6/animal), 14 dogs were allocated to one of the following five groups: spontaneous healing (SH), vehicle ointment (V), vehicle ointment + rhEGF at concentrations of 1μg/g (EGF1), 10μg/g (EGF10) or 50μg/g (EGF50). Topical administration of ointments was repeated twice per day until sacrifice at days 8 and 16. Wound area was clinically monitored. Keratinocytes proliferation (Ki67-immunolabelling), inflammatory response (IR) and areas of collagen (C) and granulation tissue (GT) were histologically measured. Kruskal-Wallis test with Dunnett correction was used for multiple group statistical comparisons. RESULTS Clinically, in comparison with SH, a significantly smaller wound area was observed in groups EGF1 and EGF10 at day 8 (p<0.05). At day 16, wound closure reached 97.8% in group EGF1 compared to 83.2% in group SH, albeit no statistically different. Histologically, at day 8, significantly more GT was observed in group EGF10 compared to all other groups (p<0.05). At day 16, in addition to a higher Ki67-immunolabelling, groups EGF1 and EGF10 demonstrated a significant decrease in GT and IR with more deposition of C compared to the other groups (p<0.05). CONCLUSION Application of rhEGF enhanced the early healing of acute oral soft tissue wounds compared to SH, predominantly at concentrations of 1 and 10μg/g. Clinic of Reconstructive Dentistry zora Ben Amara Heithem joint author Thoma Daniel S. joint author Schwarz Frank joint author Song Hyun Young joint author Capetillo Joseph joint author Koo Ki-Tae joint author Journal of Clinical Periodontology 46(1):105-117 https://www.zora.uzh.ch/id/eprint/159414/1/Amara_et_al-2018-Journal_of_Clinical_Periodontology_acc.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/159414/1/Amara_et_al-2018-Journal_of_Clinical_Periodontology_acc.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Ben Amara Heithem joint author ZORA 700 1- Thoma Daniel S. joint author ZORA 700 1- Schwarz Frank joint author ZORA 700 1- Song Hyun Young joint author ZORA 700 1- Capetillo Joseph joint author ZORA 700 1- Koo Ki-Tae joint author ZORA 773 0- Journal of Clinical Periodontology 46(1):105-117 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess