nad a22 4500 555222578 20190203201236.0 cr unu---uuuuu 190203e201809 xx s 000 0 eng 10.5167/uzh-165843 doi 10.14814/phy2.13793 doi (ZORA)oai:www.zora.uzh.ch:165843 570 ddc 30221830 pmid Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males [Elektronische Daten] [Steen Larsen, Anne-Kristine M Lundby, Sune Dandanell, Laura Oberholzer, Stefanie Keiser, Andreas B Andersen, Thomas Haider, Carsten Lundby] openAccess eu-repo Bed rest leads to impaired glucose tolerance. Whether this is linked to maladaptation's in skeletal muscle mitochondrial function and in particular to the level of reactive oxygen species (ROS) is at present unknown. The aim of this longitudinal study was to quantify skeletal muscle mitochondrial function (respiratory capacity and ROS production) together with glucose tolerance after 4days of strict bed rest in healthy young male subjects (n=14). Mitochondrial function was determined in permeabilized muscle fibers using high-resolution respirometry and fluorometry, mitochondrial content (citrate synthase [CS] activity) and antioxidant protein expression levels were assessed in parallel to this. Glucose tolerance was determined by means of oral glucose tolerance tests. Intrinsic mitochondrial respiratory capacity was augmented after the bed rest period (CI+II : 0.43±0.12 vs. 0.55±0.14 [pmol/sec/mg]/CS activity), due to a decreased CS activity (158±39 vs. 129±25mU/mg dw.). No differences were observed in ROS production (per mg of tissue or when normalized to CS activity). Furthermore, the protein content for catalase was increased while superoxide dismutase and glutathione peroxidase remained unaffected. These findings were accompanied by an impaired glucose tolerance after the bed rest period (Matsuda index: 12±6 vs. 9±5). The change in intrinsic mitochondrial respiratory capacity could be an early indication in the development of impaired glucose tolerance. The increased catalase protein content might explain that no change was seen in ROS production after 4days of bed rest. Whether these findings can be extrapolated to lifestyle-dependent decrements in physical activity and the development of type-2-diabetes remains unknown. Institute of Veterinary Physiology zora Bed rest zora glucose tolerance zora mitochondria zora reactive oxygen species production zora Larsen Steen joint author Lundby Anne-Kristine M. joint author Dandanell Sune joint author Oberholzer Laura joint author Keiser Stefanie joint author Andersen Andreas B. joint author Haider Thomas joint author Lundby Carsten joint author Physiological Reports 6(18):e13793 https://www.zora.uzh.ch/id/eprint/165843/1/Larsen_Physiological_Reports_2018_open_access.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/165843/1/Larsen_Physiological_Reports_2018_open_access.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Larsen Steen joint author ZORA 700 1- Lundby Anne-Kristine M. joint author ZORA 700 1- Dandanell Sune joint author ZORA 700 1- Oberholzer Laura joint author ZORA 700 1- Keiser Stefanie joint author ZORA 700 1- Andersen Andreas B. joint author ZORA 700 1- Haider Thomas joint author ZORA 700 1- Lundby Carsten joint author ZORA 773 0- Physiological Reports 6(18):e13793 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess