caa a22 4500 555243850 20190211030304.0 cr unu---uuuuu 190205e20180202xx s 000 0 eng 2045-2322 10.1038/s41598-018-20736-z doi (BORIS)oai:boris.unibe.ch:120751 610 ddc 29396482 pmid Gasoline particle filter reduces oxidative DNA damage in bronchial epithelial cells after whole gasoline exhaust exposure in vitro [Elektronische Daten] [Jakob Usemann, Michèle Roth, Christoph Bisig, Pierre Comte, Jan Czerwinski, Andreas C R Mayer, Philipp Latzin, Loretta Lina Müller] publishedVersion openAccess eu-repo A substantial amount of traffic-related particle emissions is released by gasoline cars, since most diesel cars are now equipped with particle filters that reduce particle emissions. Little is known about adverse health effects of gasoline particles, and particularly, whether a gasoline particle filter (GPF) influences the toxicity of gasoline exhaust emissions. We drove a dynamic test cycle with a gasoline car and studied the effect of a GPF on exhaust composition and airway toxicity. We exposed human bronchial epithelial cells (ECs) for 6 hours, and compared results with and without GPF. Two hours later, primary human natural killer cells (NKs) were added to ECs to form cocultures, while some ECs were grown as monocultures. The following day, cells were analyzed for cytotoxicity, cell surface receptor expression, intracellular markers, oxidative DNA damage, gene expression, and oxidative stress. The particle amount was significantly reduced due to GPF application. While most biological endpoints did not differ, oxidative DNA damage was significantly reduced in EC monocultures exposed to GPF compared to reference exhaust. Our findings indicate that a GPF has beneficial effects on exhaust composition and airway toxicity. Further studies are needed to assess long-term effects, also in other cell types of the lung. Usemann Jakob joint author Roth Michèle joint author Bisig Christoph joint author Comte Pierre joint author Czerwinski Jan joint author Mayer Andreas C R. joint author Latzin Philipp joint author Müller Loretta Lina joint author Scientific Reports 8(1), p. 2297 2045-2322 https://boris.unibe.ch/120751/1/Usemann_et_all_2018_Sci_Rep.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 https://boris.unibe.ch/120751/1/Usemann_et_all_2018_Sci_Rep.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- Usemann Jakob joint author BORIS 700 1- Roth Michèle joint author BORIS 700 1- Bisig Christoph joint author BORIS 700 1- Comte Pierre joint author BORIS 700 1- Czerwinski Jan joint author BORIS 700 1- Mayer Andreas C R. joint author BORIS 700 1- Latzin Philipp joint author BORIS 700 1- Müller Loretta Lina joint author BORIS 773 0- Scientific Reports 8(1), p. 2297 2045-2322 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess