naa a22 4500 555243923 20190205064556.0 cr unu---uuuuu 190205e20180704xx s 000 0 eng 1949-0976 10.1080/19490976.2017.1421888 doi (BORIS)oai:boris.unibe.ch:122995 610 ddc 29672197 pmid Inflammasomes make the case for littermate-controlled experimental design in studying host-microbiota interactions [Elektronische Daten] [Michail Mamantopoulos, Francesca Ronchi, Kathy D McCoy, Andy Wullaert] publishedVersion openAccess eu-repo Several human diseases are thought to evolve due to a combination of host genetic mutations and environmental factors that include alterations in intestinal microbiota composition termed dysbiosis. Although in some cases, host genetics may shape the gut microbiota and enable it to provoke disease, experimentally disentangling cause and consequence in such host-microbe interactions requires strict control over non-genetic confounding factors. Mouse genetic studies previously proposed Nlrp6/ASC inflammasomes as innate immunity regulators of the intestinal ecosystem. In contrast, using littermate-controlled experimental setups, we recently showed that Nlrp6/ASC inflammasomes do not alter the gut microbiota composition. Our analyses indicated that maternal inheritance and long-term separate housing are non-genetic confounders that preclude the use of non-littermate mice when analyzing host genetic effects on intestinal ecology. Here, we summarize and discuss our gut microbiota analyses in inflammasome-deficient mice for illustrating the importance of littermate experimental design in studying host-microbiota interactions. Mamantopoulos Michail joint author Ronchi Francesca joint author McCoy Kathy D. joint author Wullaert Andy joint author Gut microbes 9(4), pp. 374-381 1949-0976 https://boris.unibe.ch/122995/1/kgmi-09-04-1421888.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 https://boris.unibe.ch/122995/1/kgmi-09-04-1421888.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- Mamantopoulos Michail joint author BORIS 700 1- Ronchi Francesca joint author BORIS 700 1- McCoy Kathy D. joint author BORIS 700 1- Wullaert Andy joint author BORIS 773 0- Gut microbes 9(4), pp. 374-381 1949-0976 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess