caa a22 4500 555243990 20190211030307.0 cr unu---uuuuu 190205s2018 xx s 000 0 eng 1559-0275 10.1186/s12014-018-9217-x doi (BORIS)oai:boris.unibe.ch:123054 610 ddc 30602943 pmid Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy [Elektronische Daten] [Margaret Simonian, Dyna Shirasaki, Vivienne S. Lee, David Bervini, Michael Grace, Rachel R. Ogorzalek Loo, Joseph A. Loo, Mark P. Molloy, Marcus A. Stoodley] publishedVersion openAccess eu-repo Background Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. Methods We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach. Results Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican. Conclusion This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. Simonian Margaret joint author Shirasaki Dyna joint author Lee Vivienne S. joint author Bervini David joint author Grace Michael joint author Ogorzalek Loo Rachel R. joint author Loo Joseph A. joint author Molloy Mark P. joint author Stoodley Marcus A. joint author Clinical proteomics 15(43), p. 43 1559-0275 https://boris.unibe.ch/123054/7/s12014-018-9217-x.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 https://boris.unibe.ch/123054/7/s12014-018-9217-x.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- Simonian Margaret joint author BORIS 700 1- Shirasaki Dyna joint author BORIS 700 1- Lee Vivienne S. joint author BORIS 700 1- Bervini David joint author BORIS 700 1- Grace Michael joint author BORIS 700 1- Ogorzalek Loo Rachel R. joint author BORIS 700 1- Loo Joseph A. joint author BORIS 700 1- Molloy Mark P. joint author BORIS 700 1- Stoodley Marcus A. joint author BORIS 773 0- Clinical proteomics 15(43), p. 43 1559-0275 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess