cad a22 4500 555259374 20190312033532.0 cr unu---uuuuu 190205e20181105xx s 000 0 eng 10.5167/uzh-161624 doi 10.1128/IAI.00663-18 doi (ZORA)oai:www.zora.uzh.ch:161624 610 ddc 30396892 pmid Antibodies to protein but not glycolipid structures are important for host defense against [Elektronische Daten] [Patrick M Meyer Sauteur, Adrianus C J M de Bruijn, Catarina Graça, Anne P Tio-Gillen, Silvia C Estevão, Theo Hoogenboezem, Rudi W Hendriks, Christoph Berger, Bart C Jacobs, Annemarie M C van Rossum, Ruth Huizinga, Wendy W J Unger] openAccess eu-repo Antibody responses to Mycoplasma pneumoniae correlate with pulmonary M. pneumoniae clearance. However, M. pneumoniae-specific IgG antibodies can cross-react with the myelin glycolipid galactocerebroside (GalC) and cause neurological disorders. We assessed whether antiglycolipid antibody formation is part of the physiological immune response to M. pneumoniae We show that antibodies against M. pneumoniae proteins and glycolipids arise in serum of M. pneumoniae-infected children and mice. Although antibodies to M. pneumoniae glycolipids were mainly IgG, anti-GalC antibodies were only IgM. B-1a cells, shown to aid in protection against pathogen-derived glycolipids, are lacking in Bruton tyrosine kinase (Btk)-deficient mice. M. pneumoniae-infected Btk-deficient mice developed M. pneumoniae-specific IgG responses to M. pneumoniae proteins but not to M. pneumoniae glycolipids, including GalC. The equal recovery from M. pneumoniae infection in Btk-deficient and wild-type mice suggests that pulmonary M. pneumoniae clearance is predominantly mediated by IgG reactive with M. pneumoniae proteins and that M. pneumoniae glycolipid-specific IgG or IgM is not essential. These data will guide the development of M. pneumoniae-targeting vaccines that avoid the induction of neurotoxic antibodies. Medical Clinic zora Meyer Sauteur Patrick M. joint author de Bruijn Adrianus C J M. joint author Graça Catarina joint author Tio-Gillen Anne P. joint author Estevão Silvia C. joint author Hoogenboezem Theo joint author Hendriks Rudi W. joint author Berger Christoph joint author Jacobs Bart C. joint author van Rossum Annemarie M C. joint author Huizinga Ruth joint author Unger Wendy W J. joint author Infection and Immunity 87(2):e00663-18 https://www.zora.uzh.ch/id/eprint/161624/1/e00663-18.full.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/161624/1/e00663-18.full.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Meyer Sauteur Patrick M. joint author ZORA 700 1- de Bruijn Adrianus C J M. joint author ZORA 700 1- Graça Catarina joint author ZORA 700 1- Tio-Gillen Anne P. joint author ZORA 700 1- Estevão Silvia C. joint author ZORA 700 1- Hoogenboezem Theo joint author ZORA 700 1- Hendriks Rudi W. joint author ZORA 700 1- Berger Christoph joint author ZORA 700 1- Jacobs Bart C. joint author ZORA 700 1- van Rossum Annemarie M C. joint author ZORA 700 1- Huizinga Ruth joint author ZORA 700 1- Unger Wendy W J. joint author ZORA 773 0- Infection and Immunity 87(2):e00663-18 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess