naa a22 4500 555285561 20190206082704.0 cr unu---uuuuu 190206e201901 xx s 000 0 eng 10.3929/ethz-b-000320059 doi 10.1111/pbi.12953 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/320059 Overexpressing the H-protein of the glycine cleavage system increases biomass yield in glasshouse and field-grown transgenic tobacco plants [Elektronische Daten] [Patricia E. Lopez-Calcagno, Stuart Fisk, Kenny L. Brown, Simon E. Bull, Paul F. South, Christine A. Raines] Plant biotechnol. j. (Print) Open access ethresearch Photorespiration is essential for C3 plants, enabling oxygenic photosynthesis through the scavenging of 2‐phosphoglycolate. Previous studies have demonstrated that overexpression of the L‐ and H‐proteins of the photorespiratory glycine cleavage system results in an increase in photosynthesis and growth in Arabidopsis thaliana. Here, we present evidence that under controlled environment conditions an increase in biomass is evident in tobacco plants overexpressing the H‐protein. Importantly, the work in this paper provides a clear demonstration of the potential of this manipulation in tobacco grown in field conditions, in two separate seasons. We also demonstrate the importance of targeted overexpression of the H‐protein using the leaf‐specific promoter ST‐LS1. Although increases in the H‐protein driven by this promoter have a positive impact on biomass, higher levels of overexpression of this protein driven by the constitutive CaMV 35S promoter result in a reduction in the growth of the plants. Furthermore in these constitutive overexpressor plants, carbon allocation between soluble carbohydrates and starch is altered, as is the protein lipoylation of the enzymes pyruvate dehydrogenase and alpha‐ketoglutarate complexes. Our data provide a clear demonstration of the positive effects of overexpression of the H‐protein to improve yield under field conditions. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch photosynthesis ethresearch photorespiration ethresearch glycine decarboxylase H-protein ethresearch chlorophyll fluorescence imaging ethresearch transgenic ethresearch yield ethresearch lipoylation ethresearch Lopez-Calcagno Patricia E. joint author Fisk Stuart joint author Brown Kenny L. joint author Bull Simon E. joint author South Paul F. joint author Raines Christine A. joint author Plant biotechnology journal Wiley-Blackwell 17 (1), pp. 141-151 1467-7644 http://hdl.handle.net/20.500.11850/320059 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/320059 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Lopez-Calcagno Patricia E. joint author ETHRESEARCH 700 1- Fisk Stuart joint author ETHRESEARCH 700 1- Brown Kenny L. joint author ETHRESEARCH 700 1- Bull Simon E. joint author ETHRESEARCH 700 1- South Paul F. joint author ETHRESEARCH 700 1- Raines Christine A. joint author ETHRESEARCH 773 0- Plant biotechnology journal Wiley-Blackwell 17 (1), pp. 141-151 1467-7644 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access