cad a22 4500 555302008 20200127064818.0 cr unu---uuuuu 190206e199711 xx s 000 0 eng 10.5167/uzh-167141 doi 10.1128/CDLI.4.6.643-647.1997 doi (ZORA)oai:www.zora.uzh.ch:167141 570 ddc 630 ddc 9384282 pmid Laboratory findings in cows after experimental infection with Ehrlichia phagocytophila [Elektronische Daten] [N Pusterla, J Huder, C Wolfensberger, Ueli Braun, H Lutz] openAccess eu-repo The goal of this study was to assess various hematological variables in 10 cows after experimental infection with Ehrlichia phagocytophila. Blood samples were collected at regular intervals for examination of leukocytes for Ehrlichia organisms and for determination of hematological and biochemical variables. In addition, PCR amplification was performed throughout the disease period on blood and milk samples for the detection of E. phagocytophila organisms. The time of seroconversion and the duration of serum titers indicating positivity were determined by indirect immunofluorescence. For all cows, E. phagocytophila organisms were first detected microscopically in leukocytes 5 to 8 days postinfection and could be demonstrated for a period of 6 to 14 days. For all cows, the appearance of E. phagocytophila organisms in leukocytes coincided with transient erythropenia, leukopenia, and thrombocytopenia and a decrease in hematocrit and hemoglobin concentration. For five lactating cows, E. phagocytophila organisms were identified in leukocytes of milk samples during the acute phase of the disease, which, we believe, has not previously been reported. E. phagocytophila DNA was detected in blood samples by nested PCR from 1 to 2 days before to 2 to 12 days after the organisms were identified microscopically. In milk samples, E. phagocytophila DNA was detected for an average of 11 days. Department of Farm Animals zora Pusterla N. joint author Huder J. joint author Wolfensberger C. joint author Braun Ueli joint author Lutz H. joint author Clinical and Diagnostic Laboratory Immunology 4(6):643-647 https://www.zora.uzh.ch/id/eprint/167141/1/040643.pdf text/html WWW-Backlink auf das Repository 1 Journal Article NonPeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/167141/1/040643.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Pusterla N. joint author ZORA 700 1- Huder J. joint author ZORA 700 1- Wolfensberger C. joint author ZORA 700 1- Braun Ueli joint author ZORA 700 1- Lutz H. joint author ZORA 773 0- Clinical and Diagnostic Laboratory Immunology 4(6):643-647 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess