caa a22 4500 555311104 20200410031535.0 cr unu---uuuuu 190207e20180516xx s 000 0 eng 1746-6148 10.1186/s12917-018-1488-y doi (BORIS)oai:boris.unibe.ch:124300 590 ddc 630 ddc 29769086 pmid Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease [Elektronische Daten] [Manuel Schmidli, Bettina Fuhrer, Nadine Kurt, David Senn, Michaela Drögemüller, Ulrich Rytz, David Emmanuel Spreng, Simone Forterre] publishedVersion openAccess eu-repo BACKGROUND: Despite the importance of inflammation during the pathogenesis of cranial cruciate ligament disease (CCLD) in dogs and despite the latest knowledge suggesting a significant role of adipose tissue in osteoarthritis, the infrapatellar fat pad (IFP) was up to now mostly disregarded in veterinary investigations. In the present study, the inflammatory activity of the IFP, the main adipose structure within the stifle joint, was thoroughly investigated to evaluate its potential impact in the pathogenesis of this common disease of our canine companions. Samples of IFP, subcutaneous adipose tissue (ScAT) of the thigh and synovial fluid in both diseased (n = 36) and healthy control (n = 23) dogs were tested for their immune cell composition but also for interleukins (IL-1β, IL-6, IL-8, IL-10), degradative enzymes (MMP-1, MMP-3, MMP-13, TIMP-2, iNOS) and adipokines (leptin and adiponectin). Characterization of the immune cell composition was ascertained by fluorescence activated cell sorting. Gene expression and protein release of the inflammatory markers was determined by real RT-qPCR and ELISA. RESULTS: IFPs of dogs with CCLD had a significantly increased immune cell count with T cells (CD3) as the most abundant immune cells. T cells and macrophages (CD14) were significantly increased compared to healthy controls or corresponding ScAT. In addition, IFPs of dogs with CCLD demonstrated a significant increase on gene as well as protein level of multiple inflammatory indicators (IL-1β, IL-6, MMP-1, MMP-13) compared to the other tissues. TNFα was only increased on gene expression. Adipokine analysis showed higher secretion of adiponectin and lower leptin secretion in IFP from dogs with CCLD than from controls. In the synovial fluid from dogs with CCLD concentrations of IL-1β, MMP-1, MMP-13 as well as leptin were significantly increased compared to the synovial fluid from healthy control dogs. CONCLUSIONS: The present study indicates that the IFP is a potential contributory factor in the pathogenesis of CCLD, due to its inflammatory phenotype and the proximity within the stifle joint. To determine the extent of this possible inter-relationship, further studies need to be undertaken. Schmidli Manuel joint author Fuhrer Bettina joint author Kurt Nadine joint author Senn David joint author Drögemüller Michaela joint author Rytz Ulrich joint author Spreng David Emmanuel joint author Forterre Simone joint author BMC veterinary research 14(1), p. 161 1746-6148 https://boris.unibe.ch/124300/1/s12917-018-1488-y text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 https://boris.unibe.ch/124300/1/s12917-018-1488-y text/html WWW-Backlink auf das Repository BORIS 700 1- Schmidli Manuel joint author BORIS 700 1- Fuhrer Bettina joint author BORIS 700 1- Kurt Nadine joint author BORIS 700 1- Senn David joint author BORIS 700 1- Drögemüller Michaela joint author BORIS 700 1- Rytz Ulrich joint author BORIS 700 1- Spreng David Emmanuel joint author BORIS 700 1- Forterre Simone joint author BORIS 773 0- BMC veterinary research 14(1), p. 161 1746-6148 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess