caa a22 4500 569207762 20200911030125.0 cr unu---uuuuu 190724e201708 xx s 000 0 eng (BORIS)oai:boris.unibe.ch:125394 610 ddc The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients [Elektronische Daten] [Ewa Dolińska, Anna Skurska, Małgorzata Pietruska, Violetta Dymicka-Piekarska, Robert Milewski, Jan Pietruski, Anton Sculean] publishedVersion openAccess eu-repo The rich bacterial flora of oral cavity is controlled by innate immune response, including antibacterial peptides and among them human neutrophil peptides 1-3 (HNP1-3). The knowledge of the involvement of HNPs in innate and acquired immunity of the periodontium is fragmentary. The aim of the study was to assess alterations in HNP1-3 levels in the gingival crevicular fluid (GCF) of chronic periodontitis patients before and after nonsurgical periodontal therapy. Nineteen patients with chronic periodontitis were qualified to the study. After periodontal examination, one site with pocket depth (PD) ≥4mm was selected. All the patients received periodontal treatment involving scaling and root planing with additional systemic antibiotic therapy (Amoxicillin 375mg three times daily and Metronidazole 250mg three times daily for 7 days). Prior to therapy, 3 and 6 months after it, clinical periodontal parameters were measured and GCF was collected from previously chosen site. The level of HNP1-3 in GCF was determined by means of a commercially available enzyme-linked immunoassay kit. The periodontal therapy caused a statistically significant (p < 0.001) decrease in all the assessed clinical parameters at the sites of sample collection except for bleeding on probing. The level of HNP1-3 per measure point showed a statistically significant increase (baseline-3 months: p = 0.05, baseline-6 months: p = 0.007). Within the limits of the study, it can be stated that nonsurgical periodontal therapy with additional systemic administration of Amoxicillin and Metronidazole increases the level of HNP1-3 in GCF. Dolińska Ewa joint author Skurska Anna joint author Pietruska Małgorzata joint author Dymicka-Piekarska Violetta joint author Milewski Robert joint author Pietruski Jan joint author Sculean Anton joint author Archivum immunologiae et therapiae experimentalis 65(4), pp. 355-361 Onlinezugriff via WWW text/html Dolińska, Ewa; Skurska, Anna; Pietruska, Małgorzata; Dymicka-Piekarska, Violetta; Milewski, Robert; Pietruski, Jan; Sculean, Anton (2017). The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients. Archivum immunologiae et therapiae experimentalis, 65(4), pp. 355-361. Springer 10.1007/s00005-016-0451-5 <http://dx.doi.org/10.1007/s00005-016-0451-5> BORIS BK010053 XK010053 XK010000 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS BORIS BORIS info:eu-repo/semantics/article openAccess BORIS 856 42 Onlinezugriff via WWW text/html Dolińska, Ewa; Skurska, Anna; Pietruska, Małgorzata; Dymicka-Piekarska, Violetta; Milewski, Robert; Pietruski, Jan; Sculean, Anton (2017). The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients. Archivum immunologiae et therapiae experimentalis, 65(4), pp. 355-361. Springer 10.1007/s00005-016-0451-5 <http://dx.doi.org/10.1007/s00005-016-0451-5> BORIS 700 1- Dolińska Ewa joint author BORIS 700 1- Skurska Anna joint author BORIS 700 1- Pietruska Małgorzata joint author BORIS 700 1- Dymicka-Piekarska Violetta joint author BORIS 700 1- Milewski Robert joint author BORIS 700 1- Pietruski Jan joint author BORIS 700 1- Sculean Anton joint author BORIS 773 0- Archivum immunologiae et therapiae experimentalis 65(4), pp. 355-361