caa a22 4500 569232538 20200410094258.0 cr unu---uuuuu 190724e20170227xx s 000 0 eng 1472-6831 10.1186/s12903-017-0348-7 doi 10.1186/s12903-017-0348-7 doi (BORIS)oai:boris.unibe.ch:122916 (ZORA)oai:www.zora.uzh.ch:144973 610 ddc 28241819 pmid Response of human dental pulp cells to a silver-containing PLGA/TCP-nanofabric as a potential antibacterial regenerative pulp-capping material [Elektronische Daten] [Barbara Cvikl, Samuel C Hess, Richard J Miron, Hermann Agis, Dieter Bosshardt, Thomas Attin, Patrick R Schmidlin, Adrian Lussi] publishedVersion openAccess eu-repo BACKGROUND Damage or exposure of the dental pulp requires immediate therapeutic intervention. METHODS This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24h after stimulation. RESULTS In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72h showed similar relative BrdU incorporation than cells stimulated with Coll and Coll-HA. A prominent increase in the pro-inflammatory genes IL-6 and TNF-α was observed when cells were cultured with PLGA/Ag-TCP compared to the other groups. This increase was parallel with a slight increase in AP expression. Overall, no differences between the two culture methods were observed. CONCLUSIONS PLGA/Ag-TCP decreased viability and proliferation rate of human dental pulp cells and increased the pro-inflammatory capacity and alkaline phosphatase expression. Whether these cellular responses observed in vitro translate into pulp regeneration in vivo will be assessed in further studies. Clinic of Conservative and Preventive Dentistry zora Cvikl Barbara joint author Hess Samuel C. joint author Miron Richard J. joint author Agis Hermann joint author Bosshardt Dieter joint author Attin Thomas joint author Schmidlin Patrick R. joint author Lussi Adrian joint author BMC Oral Health 17(1), p. 57 1472-6831 https://boris.unibe.ch/122916/1/document.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 https://boris.unibe.ch/122916/1/document.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- Cvikl Barbara joint author BORIS 700 1- Hess Samuel C. joint author BORIS 700 1- Miron Richard J. joint author BORIS 700 1- Agis Hermann joint author BORIS 700 1- Bosshardt Dieter joint author BORIS 700 1- Attin Thomas joint author BORIS 700 1- Schmidlin Patrick R. joint author BORIS 700 1- Lussi Adrian joint author BORIS 773 0- BMC Oral Health 17(1), p. 57 1472-6831 ZORA 856 40 https://www.zora.uzh.ch/id/eprint/144973/1/52_OA_Attin_HumanDentalPulpCell.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Cvikl Barbara joint author ZORA 700 1- Hess Samuel C. joint author ZORA 700 1- Miron Richard J. joint author ZORA 700 1- Agis Hermann joint author ZORA 700 1- Bosshardt Dieter joint author ZORA 700 1- Attin Thomas joint author ZORA 700 1- Schmidlin Patrick R. joint author ZORA 700 1- Lussi Adrian joint author ZORA 773 0- BMC Oral Health 17(1):57 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess ZORA ZORA ZORA Journal Article openAccess