cad a22 4500 569280095 20200411095214.0 cr unu---uuuuu 190725e20190516xx s 000 0 eng 10.5167/uzh-171646 doi 10.1016/j.cell.2019.03.005 doi (ZORA)oai:www.zora.uzh.ch:171646 (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/342953 570 ddc 30982598 pmid A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer [Elektronische Daten] [Johanna Wagner, Maria Anna Rapsomaniki, Stéphane Chevrier, Tobias Anzeneder, Claus Langwieder, August Dykgers, Martin Rees, Annette Ramaswamy, Simone Muenst, Savas Deniz Soysal, Andrea Jacobs, Jonas Windhager, Karina Silina, Maries van den Broek, Konstantin Johannes Dedes, Maria Rodríguez Martínez, Walter Paul Weber, Bernd Bodenmiller] openAccess eu-repo Breast cancer is a heterogeneous disease. Tumor cells and associated healthy cells form ecosystems that determine disease progression and response to therapy. To characterize features of breast cancer ecosystems and their associations with clinical data, we analyzed 144 human breast tumor and 50 non-tumor tissue samples using mass cytometry. The expression of 73 proteins in 26 million cells was evaluated using tumor and immune cell-centric antibody panels. Tumors displayed individuality in tumor cell composition, including phenotypic abnormalities and phenotype dominance. Relationship analyses between tumor and immune cells revealed characteristics of ecosystems related to immunosuppression and poor prognosis. High frequencies of PD-L1+ tumor-associated macrophages and exhausted T cells were found in high-grade ER+ and ER− tumors. This large-scale, single-cell atlas deepens our understanding of breast tumor ecosystems and suggests that ecosystem-based patient classification will facilitate identification of individuals for precision medicine approaches targeting the tumor and its immunoenvironment. Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0 ethresearch Institute of Molecular Life Sciences zora Department of Quantitative Biomedicine zora T cell zora breast cancer zora immunosuppression zora macrophage zora mass cytometry zora single-cell analysis zora tumor ecosystem zora tumor heterogeneity zora breast cancer ethresearch tumor ecosystem ethresearch tumor heterogeneity ethresearch immunosuppression ethresearch T cell ethresearch macrophage ethresearch single-cell analysis ethresearch mass cytometry ethresearch Wagner Johanna joint author Rapsomaniki Maria Anna joint author Chevrier Stéphane joint author Anzeneder Tobias joint author Langwieder Claus joint author Dykgers August joint author Rees Martin joint author Ramaswamy Annette joint author Muenst Simone joint author Soysal Savas Deniz joint author Jacobs Andrea joint author Windhager Jonas joint author Silina Karina joint author van den Broek Maries joint author Dedes Konstantin Johannes joint author Rodríguez Martínez Maria joint author Weber Walter Paul joint author Bodenmiller Bernd joint author Cell 177(5):1330-1345 https://www.zora.uzh.ch/id/eprint/171646/1/WagnerEtAl_BreastCancerEcosystems_FinalSubmission.pdf text/html WWW-Backlink auf das Repository Onlinezugriff via WWW text/html https://www.sciencedirect.com/science/article/pii/S0092867419302673#! ZORA 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/171646/1/WagnerEtAl_BreastCancerEcosystems_FinalSubmission.pdf text/html WWW-Backlink auf das Repository ZORA 856 42 Onlinezugriff via WWW text/html https://www.sciencedirect.com/science/article/pii/S0092867419302673#! ZORA 700 1- Wagner Johanna joint author ZORA 700 1- Rapsomaniki Maria Anna joint author ZORA 700 1- Chevrier Stéphane joint author ZORA 700 1- Anzeneder Tobias joint author ZORA 700 1- Langwieder Claus joint author ZORA 700 1- Dykgers August joint author ZORA 700 1- Rees Martin joint author ZORA 700 1- Ramaswamy Annette joint author ZORA 700 1- Muenst Simone joint author ZORA 700 1- Soysal Savas Deniz joint author ZORA 700 1- Jacobs Andrea joint author ZORA 700 1- Windhager Jonas joint author ZORA 700 1- Silina Karina joint author ZORA 700 1- van den Broek Maries joint author ZORA 700 1- Dedes Konstantin Johannes joint author ZORA 700 1- Rodríguez Martínez Maria joint author ZORA 700 1- Weber Walter Paul joint author ZORA 700 1- Bodenmiller Bernd joint author ZORA 773 0- Cell 177(5):1330-1345 ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/342953 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Wagner Johanna joint author ETHRESEARCH 700 1- Rapsomaniki Maria A. joint author ETHRESEARCH 700 1- Chevrier Stéphane joint author ETHRESEARCH 700 1- Anzeneder Tobias joint author ETHRESEARCH 700 1- Langwieder Claus joint author ETHRESEARCH 700 1- Dykgers August joint author ETHRESEARCH 700 1- Rees Martin joint author ETHRESEARCH 700 1- Ramaswamy Annette joint author ETHRESEARCH 700 1- Muenst Simone joint author ETHRESEARCH 700 1- Soysal Savas D. joint author ETHRESEARCH 700 1- Jacobs Andrea joint author ETHRESEARCH 700 1- Windhager Jonas joint author ETHRESEARCH 700 1- Silina Karina joint author ETHRESEARCH 700 1- van der Broek Maries joint author ETHRESEARCH 700 1- Dedes Konstantin joint author ETHRESEARCH 700 1- Rodríguez Martínez Maria joint author ETHRESEARCH 700 1- Weber Walter P. joint author ETHRESEARCH 700 1- Bodenmiller Bernd joint author ETHRESEARCH 773 0- Cell Cambridge, MA : Cell Press 177 (5), pp. 1330-1345.e18 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access