naa a22 4500 569311705 20190727060926.0 cr unu---uuuuu 190727e20190724xx s 000 0 eng 10.3929/ethz-b-000355162 doi 10.3389/fmolb.2019.00058 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/355162 100 kHz MAS Proton-Detected NMR Spectroscopy of Hepatitis B Virus Capsids [Elektronische Daten] [Lauriane Lecoq, Maarten Schledorn, Shishan Wang, Susanne Smith-Penzel, Alexander A. Malär, Morgane Callon, Michael Nassal, Beat H. Meier, Anja Böckmann] Open access ethresearch We sequentially assigned the fully-protonated capsids made from core proteins of the Hepatitis B virus using proton detection at 100 kHz magic-angle spinning (MAS) in 0.7 mm rotors and compare sensitivity and assignment completeness to previously obtained assignments using carbon-detection techniques in 3.2 mm rotors and 17.5 kHz MAS. We show that proton detection shows a global gain of a factor ~50 in mass sensitivity, but that signal-to-noise ratios and completeness of the assignment was somewhat higher for carbon-detected experiments for comparable experimental times. We also show that deuteration and HN back protonation improves the proton linewidth at 100 kHz MAS by a factor of 1.5, from an average of 170-110 Hz, and by a factor of 1.3 compared to deuterated capsids at 60 kHz MAS in a 1.3 mm rotor. Yet, several HN protons cannot be back-exchanged due to solvent inaccessibility, which results in a total of 15% of the amides missing in the spectra. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Solid-state NMR ethresearch Fast MAS ethresearch Proton detection ethresearch Carbon detection ethresearch Deuteration ethresearch Hepatitis B virus ethresearch Capsid ethresearch Core protein ethresearch Lecoq Lauriane joint author Schledorn Maarten joint author Wang Shishan joint author Smith-Penzel Susanne joint author Malär Alexander A. joint author Callon Morgane joint author Nassal Michael joint author Meier Beat H. joint author Böckmann Anja joint author Frontiers in Molecular Biosciences Lausanne : Frontiers Media 6, p. 58 2296-889X http://hdl.handle.net/20.500.11850/355162 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/355162 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Lecoq Lauriane joint author ETHRESEARCH 700 1- Schledorn Maarten joint author ETHRESEARCH 700 1- Wang Shishan joint author ETHRESEARCH 700 1- Smith-Penzel Susanne joint author ETHRESEARCH 700 1- Malär Alexander A. joint author ETHRESEARCH 700 1- Callon Morgane joint author ETHRESEARCH 700 1- Nassal Michael joint author ETHRESEARCH 700 1- Meier Beat H. joint author ETHRESEARCH 700 1- Böckmann Anja joint author ETHRESEARCH 773 0- Frontiers in Molecular Biosciences Lausanne : Frontiers Media 6, p. 58 2296-889X BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access