cad a22 4500 56932985X 20200215084100.0 cr unu---uuuuu 190727s2019 xx s 000 0 eng 10.5167/uzh-172030 doi 10.1371/journal.pone.0215294 doi (ZORA)oai:www.zora.uzh.ch:172030 610 ddc 31059517 pmid The voxel-wise analysis of false negative fMRI activation in regions of provoked impaired cerebrovascular reactivity [Elektronische Daten] [Christiaan Hendrik Bas van Niftrik, Marco Piccirelli, Giovanni Muscas, Martina Sebök, Joseph Arnold Fisher, Oliver Bozinov, Christoph Stippich, Antonios Valavanis, Luca Regli, Jorn Fierstra] openAccess eu-repo Task-evoked Blood-oxygenation-level-dependent (BOLD-fMRI) signal activation is widely used to interrogate eloquence of brain areas. However, data interpretation can be improved, especially in regions with absent BOLD-fMRI signal activation. Absent BOLD-fMRI signal activation may actually represent false-negative activation due to impaired cerebrovascular reactivity (BOLD-CVR) of the vascular bed. The relationship between impaired BOLD-CVR and BOLD-fMRI signal activation may be better studied in healthy subjects where neurovascular coupling is known to be intact. Using a model-based prospective end-tidal carbon dioxide (CO2) targeting algorithm, we performed two controlled 3 tesla BOLD-CVR studies on 17 healthy subjects: 1: at the subjects' individual resting end-tidal CO2 baseline. 2: Around +6.0 mmHg CO2 above the subjects' individual resting baseline. Two BOLD-fMRI finger-tapping experiments were performed at similar normo- and hypercapnic levels. Relative BOLD fMRI signal activation and t-values were calculated for BOLD-CVR and BOLD-fMRI data. For each component of the cerebral motor-network (precentral gyrus, postcentral gyrus, supplementary motor area, cerebellum und fronto-operculum), the correlation between BOLD-CVR and BOLD-fMRI signal changes and t-values was investigated. Finally, a voxel-wise quantitative analysis of the impact of BOLD-CVR on BOLD-fMRI was performed. For the motor-network, the linear correlation coefficient between BOLD-CVR and BOLD-fMRI t-values were significant (p<0.01) and in the range 0.33-0.55, similar to the correlations between the CVR and fMRI Δ%signal (p<0.05; range 0.34-0.60). The linear relationship between CVR and fMRI is challenged by our voxel-wise analysis of Δ%signal and t-value change between normo- and hypercapnia. Our main finding is that BOLD fMRI signal activation maps are markedly dampened in the presence of impaired BOLD-CVR and highlights the importance of a complementary BOLD-CVR assessment in addition to a task-evoked BOLD fMRI to identify brain areas at risk for false-negative BOLD-fMRI signal activation. Clinic for Neuroradiology zora Clinic for Neurosurgery zora van Niftrik Christiaan Hendrik Bas joint author Piccirelli Marco joint author Muscas Giovanni joint author Sebök Martina joint author Fisher Joseph Arnold joint author Bozinov Oliver joint author Stippich Christoph joint author Valavanis Antonios joint author Regli Luca joint author Fierstra Jorn joint author PLoS ONE 14(5):e0215294 https://www.zora.uzh.ch/id/eprint/172030/1/10.1371journal.pone.0215294_.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/172030/1/10.1371journal.pone.0215294_.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- van Niftrik Christiaan Hendrik Bas joint author ZORA 700 1- Piccirelli Marco joint author ZORA 700 1- Muscas Giovanni joint author ZORA 700 1- Sebök Martina joint author ZORA 700 1- Fisher Joseph Arnold joint author ZORA 700 1- Bozinov Oliver joint author ZORA 700 1- Stippich Christoph joint author ZORA 700 1- Valavanis Antonios joint author ZORA 700 1- Regli Luca joint author ZORA 700 1- Fierstra Jorn joint author ZORA 773 0- PLoS ONE 14(5):e0215294 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess