New radioisotope-free method for measuring bacterial production using [15N5]-2′-deoxyadenosine and liquid chromatography mass spectrometry (LC-MS) in aquatic environments
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| 024 | 7 | 0 | |a 10.1007/s10872-015-0310-8 |2 doi |
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| 245 | 0 | 0 | |a New radioisotope-free method for measuring bacterial production using [15N5]-2′-deoxyadenosine and liquid chromatography mass spectrometry (LC-MS) in aquatic environments |h [Elektronische Daten] |c [Kenji Tsuchiya, Tomoharu Sano, Nobuyuki Kawasaki, Hideki Fukuda, Noriko Tomioka, Koji Hamasaki, Yuya Tada, Shinji Shimode, Tatsuki Toda, Akio Imai] |
| 520 | 3 | |a This study proposed a non-radioactive quantitative measurement of bacterial production using stable isotope nitrogen-15-labeled deoxyadenosine ([15N5]-2′-deoxyadenosine; 15N-dA) by liquid chromatography mass spectrometry (LC-MS). The method of preparing 5N-dA described in this study included incubation of seawater or lake water with 15N-dA for 5-24h, filtration onto a membrane filter, DNA extraction, enzymatic hydrolysis of DNA to nucleosides and quantification of 15N-dA by LC-MS. In the DNA extraction, the silica beads method was examined first, but a large amount of salts and enzymatic inhibitors used in the method caused failure of subsequent procedures, such as enzymatic hydrolysis and LC-MS analysis. On the other hand, the magnetic beads method showed much better results for the extraction. The incorporation rate of 15N-dA was significantly positively correlated to that of tritium-labeled thymidine (3H-TdR) in samples of coastal seawaters and lake waters. The average 15N-dA: 3H-TdR incorporation ratio for the seawater sample was 0.55 with 2.5 and 97.5% confidence intervals of 0.51 and 0.58, respectively; the average ratio for the lake water sample was 0.28 with 2.5 and 97.5% confidence intervals of 0.23 and 0.34, respectively. The results suggest that the 15N-dA method can be applied to the measurement of bacterial production in aquatic ecosystems, and that this method can accurately predict the DNA synthesis rates measured by the conventional method. | |
| 540 | |a The Oceanographic Society of Japan and Springer Japan, 2015 | ||
| 690 | 7 | |a Bacterial production |2 nationallicence | |
| 690 | 7 | |a Stable isotope |2 nationallicence | |
| 690 | 7 | |a Liquid chromatography mass spectrometry (LC-MS) |2 nationallicence | |
| 690 | 7 | |a DNA synthesis |2 nationallicence | |
| 690 | 7 | |a 2′-deoxyadenosine |2 nationallicence | |
| 700 | 1 | |a Tsuchiya |D Kenji |u Faculty of Science and Engineering, Soka University, 1-236, Tangi-machi, 192-8577, Hachioji, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Sano |D Tomoharu |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Kawasaki |D Nobuyuki |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Fukuda |D Hideki |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | |
| 700 | 1 | |a Tomioka |D Noriko |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Hamasaki |D Koji |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | |
| 700 | 1 | |a Tada |D Yuya |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | |
| 700 | 1 | |a Shimode |D Shinji |u Graduate School of Environment and Information Science, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Kanagawa, Japan |4 aut | |
| 700 | 1 | |a Toda |D Tatsuki |u Faculty of Science and Engineering, Soka University, 1-236, Tangi-machi, 192-8577, Hachioji, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Imai |D Akio |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | |
| 773 | 0 | |t Journal of Oceanography |d Springer Japan |g 71/6(2015-12-01), 675-683 |x 0916-8370 |q 71:6<675 |1 2015 |2 71 |o 10872 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10872-015-0310-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10872-015-0310-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tsuchiya |D Kenji |u Faculty of Science and Engineering, Soka University, 1-236, Tangi-machi, 192-8577, Hachioji, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sano |D Tomoharu |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawasaki |D Nobuyuki |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fukuda |D Hideki |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tomioka |D Noriko |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hamasaki |D Koji |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tada |D Yuya |u Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, 277-8564, Kashiwa, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shimode |D Shinji |u Graduate School of Environment and Information Science, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Kanagawa, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Toda |D Tatsuki |u Faculty of Science and Engineering, Soka University, 1-236, Tangi-machi, 192-8577, Hachioji, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Imai |D Akio |u National Institute for Environmental Studies, 16-2, Onogawa, 305-8506, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Oceanography |d Springer Japan |g 71/6(2015-12-01), 675-683 |x 0916-8370 |q 71:6<675 |1 2015 |2 71 |o 10872 | ||