caa a22 4500 605456100 CHVBK 20210128100216.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00167-014-3412-8 doi (NATIONALLICENCE)springer-10.1007/s00167-014-3412-8 Mechanostimulation changes the catabolic phenotype of human dedifferentiated osteoarthritic chondrocytes [Elektronische Daten] [Florian Halbwirth, Eugenia Niculescu-Morzsa, Hannes Zwickl, Christoph Bauer, Stefan Nehrer] Purpose: The treatment of cartilage defects with matrix-embedded autologous chondrocytes is a promising method to support the repair process and to foster reconstitution of full functionality of the joint. Methods: Human osteoarthritic chondrocytes were harvest from nine different patients (mean±SD age 68±8years) who underwent total knee replacement. The chondrocytes were embedded after a precultivation phase into a collagen I hydrogel. Mid-term intermitted mechanostimulation on matrix-embedded dedifferentiated human osteoarthritic chondrocytes was performed by intermittently applying a cyclic sinusoid compression regime for 4days (cycles of 1h of sinusoidal stimulation (1Hz) and 4h of break; maximum compression 2.5%). Stimulated (Flex) and non-stimulated (No Flex) cell matrix constructs were analysed concerning the expression of genes involved in tissue metabolism, the content of sulphated glycosaminoglycans (sGAG) and the morphology of the chondrocytes. Results: Gene expression analysis showed a high significant increase in collagen type II expression (p<0.001), a significant increase in aggrecan expression (p<0.04) and a high significant decrease in MMP-13 expression (p<0.001) under stimulation condition compared with unstimulated controls. No significant changes were found in the gene expression rate of MMP-3. This positive effect of the mechanostimulation was confirmed by the analyses of sGAG. Mechanically stimulated cell-matrix constructs had nearly tripled sGAG content than the non-stimulated control (p<0.002). In addition, histological examination showed that morphology of chondrocytes was altered from a spindle-shaped to a chondrocyte-characteristic rounded phenotype. Conclusion: Mid-term intermitted mechanical stimulation in vitro has the potential to improve the cell quality of cell matrix constructs prepared from dedifferentiated osteoarthritic chondrocytes. This observation may extend the inclusion criteria for matrix-assisted autologous chondrocyte implantation (MACI) and confirms the importance of moderate dynamic compression in clinical rehabilitation after MACI. European Society of Sports Traumatology, Knee Surgery, Arthroscopy (ESSKA), 2014 Mechanostimulation nationallicence Tissue engineering nationallicence Cell-seeded scaffold nationallicence Collagen I hydrogel nationallicence Human osteoarthritic chondrocytes nationallicence Halbwirth Florian Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut Niculescu-Morzsa Eugenia Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut Zwickl Hannes Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut Bauer Christoph Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut Nehrer Stefan Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/1(2015-01-01), 104-111 0942-2056 23:1<104 2015 23 167 https://doi.org/10.1007/s00167-014-3412-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-014-3412-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Halbwirth Florian Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut NATIONALLICENCE 700 1- Niculescu-Morzsa Eugenia Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut NATIONALLICENCE 700 1- Zwickl Hannes Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut NATIONALLICENCE 700 1- Bauer Christoph Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut NATIONALLICENCE 700 1- Nehrer Stefan Center for Regenerative Medicine and Orthopedics, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/1(2015-01-01), 104-111 0942-2056 23:1<104 2015 23 167