Effect of two different preparations of platelet-rich plasma on synoviocytes
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| 024 | 7 | 0 | |a 10.1007/s00167-014-3113-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00167-014-3113-3 | ||
| 245 | 0 | 0 | |a Effect of two different preparations of platelet-rich plasma on synoviocytes |h [Elektronische Daten] |c [Elisa Assirelli, Giuseppe Filardo, Erminia Mariani, Elizaveta Kon, Alice Roffi, Franca Vaccaro, Maurilio Marcacci, Andrea Facchini, Lia Pulsatelli] |
| 520 | 3 | |a Purpose: To analyse the modifications induced by two different platelet-rich plasma (PRP) preparations on osteoarthritis (OA) synoviocytes, by documenting changes in gene expression of factors involved in joint physiopathology. Methods: OA synoviocytes were cultured for 7days in medium with different concentrations of either P-PRP (a pure platelet concentrate without leucocytes but with a limited number of platelets), L-PRP (a higher platelet concentrate containing leucocytes) or platelet-poor plasma (PPP). Gene expression of interleukin (IL)-1beta, IL-6, IL-8/CXCL8, tumour necrosis factor alpha, IL-10, IL-4, IL-13, metalloproteinase-13, tissue inhibitor of metalloproteinase (TIMP)-1, (TIMP)-3, (TIMP)-4, vascular endothelial growth factor, transforming growth factor beta1, fibroblast growth factor (FGF)-2, hepatocyte growth factor (HGF), hyaluronic acid (HA) synthases (HAS)-1, (HAS)-2, and (HAS)-3 was analysed by RT-PCR. HA production was determined in culture supernatants by ELISA. Results: IL-1β, IL-8 and FGF-2 were significantly induced by L-PRP compared to both P-PRP and PPP; HGF was down-modulated by L-PRP versus both P-PRP and PPP, and an inverse dose-response influence was shown for all preparations. Expression level of TIMP-4 was lower in the presence of L-PRP compared with P-PRP. HA production and HAS gene expression did not seem to be modulated by PRP. Conclusions: L-PRP is able to sustain the up-regulation of proinflammatory factors, (IL-1beta, IL-8 and FGF-2), together with a down-modulation of HGF and TIMP-4 expression, two factors that have been recognized as anti-catabolic mediators in cartilage, thus supporting the need to further optimize the PRP preparations to be applied in clinical practice. | |
| 540 | |a The Author(s), 2014 | ||
| 690 | 7 | |a Osteoarthritis |2 nationallicence | |
| 690 | 7 | |a Synoviocytes |2 nationallicence | |
| 690 | 7 | |a Platelet-rich plasma |2 nationallicence | |
| 690 | 7 | |a Soluble factors |2 nationallicence | |
| 700 | 1 | |a Assirelli |D Elisa |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 700 | 1 | |a Filardo |D Giuseppe |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | |
| 700 | 1 | |a Mariani |D Erminia |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 700 | 1 | |a Kon |D Elizaveta |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | |
| 700 | 1 | |a Roffi |D Alice |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | |
| 700 | 1 | |a Vaccaro |D Franca |u Immunohematology and Transfusion Medicine Service, San Pietro Hospital, Via Cassia 600, Rome, Italy |4 aut | |
| 700 | 1 | |a Marcacci |D Maurilio |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | |
| 700 | 1 | |a Facchini |D Andrea |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 700 | 1 | |a Pulsatelli |D Lia |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 773 | 0 | |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/9(2015-09-01), 2690-2703 |x 0942-2056 |q 23:9<2690 |1 2015 |2 23 |o 167 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00167-014-3113-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00167-014-3113-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Assirelli |D Elisa |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Filardo |D Giuseppe |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mariani |D Erminia |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kon |D Elizaveta |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Roffi |D Alice |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vaccaro |D Franca |u Immunohematology and Transfusion Medicine Service, San Pietro Hospital, Via Cassia 600, Rome, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marcacci |D Maurilio |u Laboratory of Biomechanics and Technology Innovation/NABI, 2nd Orthopaedic and Traumatologic Clinic, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Facchini |D Andrea |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pulsatelli |D Lia |u Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/9(2015-09-01), 2690-2703 |x 0942-2056 |q 23:9<2690 |1 2015 |2 23 |o 167 | ||