caa a22 4500 605457654 CHVBK 20210128100224.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00167-014-3276-y doi (NATIONALLICENCE)springer-10.1007/s00167-014-3276-y Collagen fibre and fibril ultrastructural arrangement of the superficial medial collateral ligament in the human knee [Elektronische Daten] [Stefano Zaffagnini, Giulio Marcheggiani Muccioli, Marco Franchi, Beatrice Bacchelli, Alberto Grassi, Patrizia Agati, Marilisa Quaranta, Maurilio Marcacci, Viviana De Pasquale] Purpose: The aim of the study was to investigate the collagen fibre ultrastructural arrangement and collagen fibril diameters in the superficial medial collateral ligament (sMCL) in the human knee. Considering sMCL's distinctive functions at different angles of knee flexion, it was hypothesized a significant difference between the collagen fibril diameters of each portion of the sMCL. Methods: Fourteen sMCL from seven fresh males (by chance because of the availability) cadavers (median age 40years, range 34-59years) were harvested within 12h of death. sMCLs were separated into two orders of regions for analysis. The first order (divisions) was anterior, central and posterior. Thereafter, each division was split into three regions (femoral, intermediate and tibial), generating nine portions. One sMCL from each cadaver was used for transmission electron microscopy (TEM) and morphometric analyses, whereas the contralateral sMCL was processed for light microscopy (LM) or scanning electron microscopy (SEM). Results: LM and SEM analyses showed a complex tridimensional architecture, with the presence of wavy collagen fibres or crimps. TEM analysis showed significant differences in median collagen fibril diameter among portions inside the anterior, central and posterior division of the sMCL (p<0.0001 within each division). Significant differences were also present among the median [interquartile range] collagen fibril diameters of anterior (39.4 [47.8-32.9]), central (38.5 [44.4-34.0]) and posterior (41.7 [52.2-35.4]) division (p=0.0001); femoral (38.2 [45.0-32.7]), intermediate (40.3 [47.3-36.1]) and tibial (40.7 [55.0-32.2]) region (p=0.0001). Conclusions: Human sMCL showed a complex architecture that allows restraining different knee motions at different angles of knee flexion. The posterior division of sMCL accounted for the largest median collagen fibril diameter. The femoral region of sMCL accounted for the smallest median collagen fibril diameter. The presence of crimps in the medial collateral ligament, previously identified in the rat, was confirmed in humans (taking into consideration differences between these two species). European Society of Sports Traumatology, Knee Surgery, Arthroscopy (ESSKA), 2014 Knee nationallicence Human nationallicence Medial collateral ligament nationallicence Ultrastructure nationallicence Collagen fibre array nationallicence Crimps nationallicence Zaffagnini Stefano Istituto Ortopedico Rizzoli, Bologna, Italy aut Marcheggiani Muccioli Giulio Istituto Ortopedico Rizzoli, Bologna, Italy aut Franchi Marco Faculty of Sport Sciences, University of Bologna, Bologna, Italy aut Bacchelli Beatrice Institute of Human Anatomy, University of Bologna, Bologna, Italy aut Grassi Alberto Istituto Ortopedico Rizzoli, Bologna, Italy aut Agati Patrizia Statistical Science Department, University of Bologna, Bologna, Italy aut Quaranta Marilisa Institute of Human Anatomy, University of Bologna, Bologna, Italy aut Marcacci Maurilio Istituto Ortopedico Rizzoli, Bologna, Italy aut De Pasquale Viviana Institute of Human Anatomy, University of Bologna, Bologna, Italy aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/12(2015-12-01), 3674-3682 0942-2056 23:12<3674 2015 23 167 https://doi.org/10.1007/s00167-014-3276-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-014-3276-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zaffagnini Stefano Istituto Ortopedico Rizzoli, Bologna, Italy aut NATIONALLICENCE 700 1- Marcheggiani Muccioli Giulio Istituto Ortopedico Rizzoli, Bologna, Italy aut NATIONALLICENCE 700 1- Franchi Marco Faculty of Sport Sciences, University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Bacchelli Beatrice Institute of Human Anatomy, University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Grassi Alberto Istituto Ortopedico Rizzoli, Bologna, Italy aut NATIONALLICENCE 700 1- Agati Patrizia Statistical Science Department, University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Quaranta Marilisa Institute of Human Anatomy, University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Marcacci Maurilio Istituto Ortopedico Rizzoli, Bologna, Italy aut NATIONALLICENCE 700 1- De Pasquale Viviana Institute of Human Anatomy, University of Bologna, Bologna, Italy aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/12(2015-12-01), 3674-3682 0942-2056 23:12<3674 2015 23 167