caa a22 4500 605457913 CHVBK 20210128100226.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00167-013-2697-3 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2697-3 Peri-tunnel bone loss: does it affect early tendon graft to bone tunnel healing after ACL reconstruction? [Elektronische Daten] [Pauline Lui, Yuk Lee, Tsui Mok, Yau Cheuk] Purpose: The clinical relevance and mechanisms of local bone loss early post-anterior cruciate ligament (ACL) reconstruction remain unclear. The early spatial and temporal changes of peri-tunnel bone, its molecular mechanisms and its relationships with graft-bone tunnel healing were investigated in a 12-week-old rat model. Methods: At various times, the reconstructed ACL complex was harvested for vivaCT imaging, biomechanical test, histology and immunohistochemical staining of CD68+ cells (a monocyte-macrophage lineage marker), MMP1 and MMP13. Results: The peri-tunnel bone resorbed simultaneously with improvement of graft-bone tunnel healing. There were 30.1±17.4, 46.8±10.5 and 81.5±12.3% loss of peri-tunnel BMD as well as 43.2±21.7, 78.7±8.5 and 92.4±17.7% loss of peri-tunnel bone volume/total volume (BV/TV) at week 6 at the distal femur, epiphysis and metaphysis of tibia, respectively. MMP1, MMP13 and CD68+ cells were expressed at the graft-bone tunnel interface and peri-tunnel bone and increased with time post-reconstruction at the tibia. The ultimate load and stiffness of the healing complex positively correlated with tibial tunnel bone formation and negatively correlated with tibial peri-tunnel bone. Tunnel BV/TV at the tibial metaphysis and epiphysis showed the highest correlation with ultimate load (ρ=0.591; p=0.001) and stiffness (ρ=0.427; p=0.026) of the complex, respectively. Conclusion: There was time-dependent loss of peri-tunnel bone early post-reconstruction, with the greatest loss occurring at the tibial metaphysis. This was consistent with high expression of MMP1, MMP13 and CD68+ cells at the graft-bone tunnel interface and the peri-tunnel region. The significant loss of peri-tunnel bone, though not critically affecting early tunnel healing, suggested the need to protect the knee joint early post-reconstruction. Springer-Verlag Berlin Heidelberg, 2013 Anterior cruciate ligament (ACL) reconstruction nationallicence Peri-tunnel bone loss nationallicence Tunnel healing nationallicence MMP nationallicence Monocyte-macrophage lineage marker nationallicence Lui Pauline Headquarter, Hospital Authority, 9/F, Rumsey Street Multi-Storey Carpark Building, 2 Rumsey Street, Hong Kong SAR, China aut Lee Yuk Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut Mok Tsui Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut Cheuk Yau Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/3(2015-03-01), 740-751 0942-2056 23:3<740 2015 23 167 https://doi.org/10.1007/s00167-013-2697-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2697-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lui Pauline Headquarter, Hospital Authority, 9/F, Rumsey Street Multi-Storey Carpark Building, 2 Rumsey Street, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Lee Yuk Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Mok Tsui Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Cheuk Yau Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/3(2015-03-01), 740-751 0942-2056 23:3<740 2015 23 167