caa a22 4500 605458979 CHVBK 20210128100231.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00167-013-2820-5 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2820-5 Assessment of essential characteristics of two different scaffolds for tendon in situ regeneration [Elektronische Daten] [Markus Wagenhäuser, Matthias Pietschmann, Denitsa Docheva, Mehmet Gülecyüz, Volkmar Jansson, Peter Müller] Purpose: Rotator cuff tears are challenging as the rate of re-ruptures remains high. Thus, new therapeutic strategies need to be developed. Tendon in situ regeneration (TSR) attempts to produce cell-scaffold constructs in vitro, which can produce tendinous tissue of high quality after replantation. Therefore, it is essential to find suitable scaffolds that can provide acceptable biofunctionality and biocompatibility. This study compares characteristics of scaffolds for in situ regeneration: a polyglycolic acid/PDS scaffold (PP-sca) (Ethisorb, Ethicon, Germany) and a collagen sponge (col-spo) (TissueTek, Germany) with a basal strengthening membrane. Methods: Tendon-derived cells (TDCs) were isolated from the long head of the biceps tendon. Gene expression for collagen type I, collagen type III, decorin, scleraxis and tenomodulin was analysed in the third cell passage. Cell proliferation in cell seeded scaffolds was tested using a WST-1 assay. In addition, the tensile strength of both scaffolds was measured using a universal-testing machine (Zwick/Roell, Ulm, Germany). Results: The results from this study indicate a genotypic drift during the in vitro cultivation of the TDCs. The PP-sca showed good biofunctional results, including low initial loss of cells after cell seeding. The proliferation rates were approximately equal in each type of scaffold. The col-spo provided superior tensile strength compared with the PP-sca (p<0.01). Conclusion: Overall, the col-spo seems to be more suitable for TSR. It may become a clinical alternative in the future to achieve more satisfying results, concerning function and pain. Level of evidence: Experimental study/case series, Level IV. Springer-Verlag Berlin Heidelberg, 2014 PGA nationallicence Scaffold nationallicence Collagen nationallicence Tendon tissue engineering nationallicence Tendon nationallicence Rotator cuff nationallicence Tear nationallicence Wagenhäuser Markus Department of Vascular and Endovascular Surgery, Heinrich-Heine University Düsseldorf, Moorenstraße 5, 40225, Düsseldorf, Germany aut Pietschmann Matthias Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut Docheva Denitsa Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University, Campus Innenstadt, Nußbaumstr. 20, 80336, Munich, Germany aut Gülecyüz Mehmet Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut Jansson Volkmar Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut Müller Peter Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/4(2015-04-01), 1239-1246 0942-2056 23:4<1239 2015 23 167 https://doi.org/10.1007/s00167-013-2820-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2820-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wagenhäuser Markus Department of Vascular and Endovascular Surgery, Heinrich-Heine University Düsseldorf, Moorenstraße 5, 40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Pietschmann Matthias Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Docheva Denitsa Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University, Campus Innenstadt, Nußbaumstr. 20, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Gülecyüz Mehmet Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Jansson Volkmar Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Müller Peter Department of Orthopaedic Surgery, Ludwig-Maximilians-University Munich, Campus Grosshadern, Marchioninistr. 15, 81377, Munich, Germany aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/4(2015-04-01), 1239-1246 0942-2056 23:4<1239 2015 23 167