caa a22 4500 605459290 CHVBK 20210128100233.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00167-014-2897-5 doi (NATIONALLICENCE)springer-10.1007/s00167-014-2897-5 In vivo monitoring of angiogenesis during tendon repair: a novel MRI-based technique in a rat patellar tendon model [Elektronische Daten] [Richard Stange, Hacer Sahin, Britta Wieskötter, Thorsten Persigehl, Janine Ring, Christoph Bremer, Michael Raschke, Volker Vieth] Purpose: Recent in vivo studies were able to show the impairing effect of neoangiogenesis in degenerative tendon diseases. Clinical in vivo monitoring of angiogenesis in injured tendons therefore seems to be crucial for an accurate therapeutic approach. The aim of this study was to develop a novel magnetic resonance imaging (MRI)-based technique for observing angiogenesis during tendon healing in vivo. Methods: Tendinopathy was induced by an in situ freezing model of rat patellar tendon and monitored after 7, 14, and 28days. Animals were randomly divided into an imaging and immunohistochemical group. MRI with a ‘blood pool' contrast agent was used to determine neoangiogenesis during tendon healing. MRI was compared to histochemical staining and quantification of blood vessels in injured and native tendons. Results: MRI data revealed a peak in changes in the transverse relaxation rate (ΔR 2*), which is proportional to relative blood volume, 7days after surgery and decrease until day 28. Histological microvessel density and vascular endothelial growth factor synthesis were also most evident at day 7 and decreased over time. Conclusions: The current results are demonstrating a time-dependent correlation between microvessel density and ΔR 2*. Thus, MRI-based evaluation of angiogenesis in the tendon might be a new promising technique for in vivo monitoring of angiogenesis and therapy response in the future. Springer-Verlag Berlin Heidelberg, 2014 Tendon healing nationallicence Angiogenesis nationallicence MRI nationallicence USPIO nationallicence Relaxometry nationallicence Stange Richard Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut Sahin Hacer Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut Wieskötter Britta Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut Persigehl Thorsten Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut Ring Janine Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut Bremer Christoph Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut Raschke Michael Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut Vieth Volker Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/8(2015-08-01), 2433-2439 0942-2056 23:8<2433 2015 23 167 https://doi.org/10.1007/s00167-014-2897-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-014-2897-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stange Richard Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Sahin Hacer Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Wieskötter Britta Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Persigehl Thorsten Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Ring Janine Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Bremer Christoph Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Raschke Michael Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 700 1- Vieth Volker Department of Clinical Radiology, University Hospital Münster, Münster, Germany aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/8(2015-08-01), 2433-2439 0942-2056 23:8<2433 2015 23 167