PVA-chitosan composite hydrogel versus alginate beads as a potential mesenchymal stem cell carrier for the treatment of focal cartilage defects
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| 024 | 7 | 0 | |a 10.1007/s00167-013-2723-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00167-013-2723-5 | ||
| 245 | 0 | 0 | |a PVA-chitosan composite hydrogel versus alginate beads as a potential mesenchymal stem cell carrier for the treatment of focal cartilage defects |h [Elektronische Daten] |c [Havva Dashtdar, Malliga Murali, Azlina Abbas, Abdulrazzaq Suhaeb, Lakshmi Selvaratnam, Liang Tay, Tunku Kamarul] |
| 520 | 3 | |a Purpose: To investigate whether mesenchymal stem cells (MSCs) seeded in novel polyvinyl alcohol (PVA)-chitosan composite hydrogel can provide comparable or even further improve cartilage repair outcomes as compared to previously established alginate-transplanted models. Methods: Medial femoral condyle defect was created in both knees of twenty-four mature New Zealand white rabbits, and the animals were divided into four groups containing six animals each. After 3weeks, the right knees were transplanted with PVA-chitosan-MSC, PVA-chitosan scaffold alone, alginate-MSC construct or alginate alone. The left knee was kept as untreated control. Animals were killed at the end of 6months after transplantation, and the cartilage repair was assessed through Brittberg morphological score, histological grading by O'Driscoll score and quantitative glycosaminoglycan analysis. Results: Morphological and histological analyses showed significant (p<0.05) tissue repair when treated with PVA-chitosan-MSC or alginate MSC as compared to the scaffold only and untreated control. In addition, safranin O staining and the glycosaminoglycan (GAG) content were significantly higher (p<0.05) in MSC treatment groups than in scaffold-only or untreated control group. No significant difference was observed between the PVA-chitosan-MSC- and alginate-MSC-treated groups. Conclusion: PVA-chitosan hydrogel seeded with mesenchymal stem cells provides comparable treatment outcomes to that of previously established alginate-MSC construct implantation. This study supports the potential use of PVA-chitosan hydrogel seeded with MSCs for clinical use in cartilage repair such as traumatic injuries. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2013 | ||
| 690 | 7 | |a PVA-chitosan |2 nationallicence | |
| 690 | 7 | |a Mesenchymal stem cell |2 nationallicence | |
| 690 | 7 | |a Tissue engineering |2 nationallicence | |
| 690 | 7 | |a Cartilage repair |2 nationallicence | |
| 690 | 7 | |a Biomaterials |2 nationallicence | |
| 700 | 1 | |a Dashtdar |D Havva |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 700 | 1 | |a Murali |D Malliga |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 700 | 1 | |a Abbas |D Azlina |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 700 | 1 | |a Suhaeb |D Abdulrazzaq |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 700 | 1 | |a Selvaratnam |D Lakshmi |u School of Medicine and Health Sciences, Monash University, Sunway Campus, Selangor, Malaysia |4 aut | |
| 700 | 1 | |a Tay |D Liang |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 700 | 1 | |a Kamarul |D Tunku |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | |
| 773 | 0 | |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/5(2015-05-01), 1368-1377 |x 0942-2056 |q 23:5<1368 |1 2015 |2 23 |o 167 | |
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00167-013-2723-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dashtdar |D Havva |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Murali |D Malliga |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abbas |D Azlina |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Suhaeb |D Abdulrazzaq |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Selvaratnam |D Lakshmi |u School of Medicine and Health Sciences, Monash University, Sunway Campus, Selangor, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tay |D Liang |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kamarul |D Tunku |u Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Lembah Pantai, Kuala Lumpur, Malaysia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/5(2015-05-01), 1368-1377 |x 0942-2056 |q 23:5<1368 |1 2015 |2 23 |o 167 | ||