caa a22 4500 605459711 CHVBK 20210128100235.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00167-013-2719-1 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2719-1 Synovial cytokine expression in ankle osteoarthritis depends on age and stage [Elektronische Daten] [Hagen Schmal, Ralf Henkelmann, Alexander Mehlhorn, Kilian Reising, Gerrit Bode, Norbert Südkamp, Philipp Niemeyer] Purpose: Aim of the study was the analysis of cytokine expression in ankle osteoarthritis (OA) hypothesizing age-dependent regulation patterns. Methods: Forty-nine patients undergoing an arthroscopy of the ankle with different stages of chronic OA were prospectively included in a clinical trial comparing the group <18years (n=9, Ø15.1±2.0years) with the older patients (≥18years, n=40, Ø36.5±11.9). Lavage fluids were analysed by ELISA for levels of aggrecan, BMP-2/7, IGF-1/R, bFGF, CD105, MMP-13, and IL-1β. Additionally, clinical parameters and scores (FFI, CFSS, AOFAS) were evaluated and supplemented by radiographic scores [Kellgren-Lawrence Score (KLS) for conventional X-rays, Ankle Osteoarthritis Scoring System (AOSS) for MRI]. Results: In contrast to distribution of gender and BMI (p<0.005), parameters characterizing the cartilage defect as ICRS grading, size, and duration of symptoms were not dependent on age. The incidence of osteochondritis dissecans (OCD) was higher in the group <18years (p<0.006), but the average degree of OCD grading was not different. KLS and AOSS were significantly higher in the group ≥18years (p<0.02). Correlating with the higher degree of OA in the elderly, clinical function measured by FFI and AOFAS was statistically significantly worse (p<0.05). Intra-articular concentrations of aggrecan (3.1-fold), bFGF (8.7-fold), BMP-7 (2.7-fold), and CD105 (1.5-fold) were statistically significantly higher in the group≥18years (p<0.03). Conclusions: Confirming the hypothesis, increased synovial levels of aggrecan, bFGF, BMP-7, and CD105 were found in patients over 18years. This correlated with a higher stage of OA determined by radiographic changes or deteriorated function and may offer starting points for new diagnostics and interventional strategies. Level of evidence: II. Springer-Verlag Berlin Heidelberg, 2013 Ankle nationallicence Osteoarthritis nationallicence Ankle Osteoarthritis Scoring System nationallicence CD105 nationallicence BMP-7 nationallicence Aggrecan nationallicence Biomarker nationallicence bFGF nationallicence Clinical trial nationallicence Schmal Hagen Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Henkelmann Ralf Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Mehlhorn Alexander Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Reising Kilian Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Bode Gerrit Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Südkamp Norbert Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Niemeyer Philipp Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1359-1367 0942-2056 23:5<1359 2015 23 167 https://doi.org/10.1007/s00167-013-2719-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2719-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmal Hagen Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Henkelmann Ralf Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Mehlhorn Alexander Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Reising Kilian Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Bode Gerrit Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Südkamp Norbert Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 700 1- Niemeyer Philipp Department of Orthopaedic Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg i.Br., Germany aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1359-1367 0942-2056 23:5<1359 2015 23 167