caa a22 4500 605459797 CHVBK 20210128100235.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00167-013-2785-4 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2785-4 Effect of VEGF-A165 addition on the integration of a cortical allograft in a tibial segmental defect in rabbits [Elektronische Daten] [Miguel Ruiz-Ibán, Fausto Gonzalez-Lizán, Jorge Diaz-Heredia, Maria Elías-Martin, Carlos Correa Gorospe] Purpose: Long-bone segmental defects caused by infection, fracture, or tumour are a challenge for orthopaedic surgeons. Structural allografts are sometimes used in their treatment but their poor biological characteristics are a liability. The objective of this study was to determine whether the addition of recombinant vascular endothelial growth factor-A (VEGF) to a structural allograft improved its integration into a rabbit tibial segmental defect in a non-union model. Methods: Tibial segmental defects were filled with heat sterilized allogenic tubular tibiae sections and then stabilized with a screw plate. In the VEGF treatment group (n=6 tibiae), 2μg of VEGF added to a 50μl matrigel solution was inserted into the allograft cavity. In the control group (n=6 tibiae), only matrigel was added. After 12weeks, macroscopic and microscopic analysis, radiographs, and computerized micro-tomography (micro-CT) were performed. If allograft consolidation was present, a torsional resistance analysis was performed. Results: Addition of VEGF to the allograft decreased the rate of osteosynthesis failure compared with the control group (1/6 vs. 5/6, p=0.08), increased trabecular continuity evaluated by micro-CT in the bone-allograft interphases (8/12 vs. 2/12, p=0.036) and histological trabecular continuity (7/12 vs. 0/12, p=0.0046). Full consolidation was observed in three tibiae of the VEGF group and one in the control group (differences not significant); however, torsional resistance showed no significant differences (n.s.). Conclusion: Addition of VEGF to a structural allograph inserted into a rabbit tibial segmental defect increased allograft integration rate. Further research in this direction might help clinicians in dealing with large bone defects. Springer-Verlag Berlin Heidelberg, 2013 Tibia nationallicence Allograft nationallicence Non-union nationallicence Rabbit nationallicence VEGF nationallicence Ruiz-Ibán Miguel Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut Gonzalez-Lizán Fausto Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut Diaz-Heredia Jorge Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut Elías-Martin Maria Department of Anesthesiology and Reanimation, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut Correa Gorospe Carlos Experimental Surgery Unit, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1393-1400 0942-2056 23:5<1393 2015 23 167 https://doi.org/10.1007/s00167-013-2785-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2785-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruiz-Ibán Miguel Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Gonzalez-Lizán Fausto Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Diaz-Heredia Jorge Department of Orthopaedic Surgery and Trauma, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Elías-Martin Maria Department of Anesthesiology and Reanimation, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Correa Gorospe Carlos Experimental Surgery Unit, Hospital Universitario Ramón y Cajal, Cta Colmenar Km 9.100, 28034, Madrid, Spain aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1393-1400 0942-2056 23:5<1393 2015 23 167