caa a22 4500 605459827 CHVBK 20210128100235.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00167-013-2631-8 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2631-8 Efficacy of common surgical compounds in preventing articular chondrocyte death from desiccation [Elektronische Daten] [A. Von Keudell, H. Syed, J. Canseco, A. Gomoll] Purpose: Purpose of this study was to identify potential substances that prevent desiccation of chondrocytes. Methods: Macroscopically normal bovine cartilage explants (n=80) were exposed to room air, or covered with surgical lubricant, Lactated ringer (LR) or Seprafilm (Genzyme Biosurgery, Cambridge, MA) for 0, 30, 60 or 120min. The viability of superficial chondrocytes was measured after 48h of incubation in tissue culture media at 37°C by Live/Dead staining. Chondrotoxicity was measured as the extent of cell death below the articular surface. Statistical analysis was performed with a two-way analysis of variance on the data set and a subsequent Tukey's post hoc test. Results: Chondrocyte death correlated positively with the length of exposure, regardless of the treatment (p<0.0001). The extent of superficial chondrocyte death was minimally lower in the LR (89.1±2.6%, 80.8±1.2%) and surgical lube (84.3±1.8%, 75.9±2.7%) groups than the control (82±5.7%, 65.6±13.3%) and Seprafilm group (77.6±3.9%, 63.3±6.9%) (p<0.001) at the first two time points, with no significant difference between the latter groups. After 60 and 120min, surgical lube resulted in less chondrocyte death than all other groups (70.4±6.8% and 60.9±5.9%, all p<0.0001). Conclusion: The data suggest that depending on the expected length of exposure of the articular cartilage surface, different compounds appear to be protective. For exposures exceeding 60min, surgical lubricant demonstrated the highest protective potential. Results from this study indicate that protecting exposed articular surfaces with surgical lubricant for orthopaedic procedures lasting more than 1h lead to decreased chondrocyte death and suggest improved cartilage functional outcomes postoperatively. Springer-Verlag Berlin Heidelberg, 2013 Chondrotoxicity nationallicence Desiccation nationallicence Surgical compounds nationallicence Arthrotomies nationallicence Cartilage nationallicence Von Keudell A. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut Syed H. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut Canseco J. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut Gomoll A. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1346-1350 0942-2056 23:5<1346 2015 23 167 https://doi.org/10.1007/s00167-013-2631-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2631-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Von Keudell A. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut NATIONALLICENCE 700 1- Syed H. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut NATIONALLICENCE 700 1- Canseco J. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut NATIONALLICENCE 700 1- Gomoll A. Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1346-1350 0942-2056 23:5<1346 2015 23 167